2-Iminobiotin (Synonyms: Guanidinobiotin) |
| Catalog No.GC16664 |
iNOS and nNOS inhibitor
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 13395-35-2
Sample solution is provided at 25 µL, 10mM.
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2-Iminobiotin is a novel, potent, selective inhibitor of neuronal and inducible nitric oxide synthase.
Nitric oxide synthases (NOS) are a family of enzymes catalyzing the five-electron oxidation of L-arginine to citrulline and nitric oxide. NO produced by NOS is an important cellular signaling molecule implicated in modulating vascular tone, airway tone, insulin secretion, and peristalsis, angiogenesis and neural development [2].
In vitro: 2-Iminobiotin is a reversible inhibitor of murine iNOS and rat n-cNOS with the Ki values of 21.8 and 37.5 μM, respectively. The guanidino group of 2-iminobiotin is essential for binding. 2-iminobiotin carboxy derivatives were also inhibitors of iNOS which indicates that the free carboxyl group is not required for binding.
In vivo: A dose of 30 mg/kg per day of 2-iminobiotin after hypoxia-ischemia resulted in significant long-term neuro-protection [3]. 2-iminobiotin (2-IB) reduced hypoxia-ischemia (HI)-induced brain damage in neonatal rats. In P7 rat pups, 2-Iminobiotin treatment reduced long-term brain damage in female but not male rats [4].
References:
[1] Sup S J, Green B G, Grant S K. 2-Iminobiotin is an inhibitor of nitric oxide synthases[J]. Biochemical and biophysical research communications, 1994, 204(2): 962-968.
[2] Moncada S, Palmer R M L, Higgs E A. Nitric oxide: physiology, pathophysiology, and pharmacology[J]. Pharmacological reviews, 1991, 43(2): 109-142.
[3] Tweel E R W, van Bel F, Kavelaars A, et al. Long-term neuroprotection with 2-iminobiotin, an inhibitor of neuronal and inducible nitric oxide synthase, after cerebral hypoxia-ischemia in neonatal rats[J]. Journal of Cerebral Blood Flow & Metabolism, 2005, 25(1): 67-74.
[4] Nijboer C H A, Groenendaal F, Kavelaars A, et al. Gender-Specific Neuroprotection by 2-Iminobiotin after Hypoxia—Ischemia in the Neonatal Rat via a Nitric Oxide Independent Pathway[J]. Journal of Cerebral Blood Flow & Metabolism, 2007, 27(2): 282-292.
| Cas No. | 13395-35-2 | SDF | |
| Synonyms | Guanidinobiotin | ||
| Chemical Name | (3aR,6S,6aS)-2-amino-3a,4,6,6a-tetrahydro-1H-thieno[3,4-d]imidazole-6-pentanoic acid | ||
| Canonical SMILES | [H][C@](NC1=N)(CS[C@H]2CCCCC(O)=O)[C@@]2(N1)[H] | ||
| Formula | C10H17N3O2S | M.Wt | 243.3 |
| Solubility | DMSO : 12.5 mg/mL (51.37 mM; Need ultrasonic) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 4.1102 mL | 20.5508 mL | 41.1015 mL |
| 5 mM | 0.822 mL | 4.1102 mL | 8.2203 mL |
| 10 mM | 0.411 mL | 2.0551 mL | 4.1102 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 4 reference(s) in Google Scholar.)
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