3,3'-Dihexyloxacarbocyanine iodide (DiOC6(3) iodide) (Synonyms: DiOC6(3), NK 2280) |
Catalog No.GC33493 |
3,3'-Dihexyloxacarbocyanine iodide (DiOC6(3) iodide) is a carbocyanine dye which can be used to monitor changes in mitochondrial membrane potential.
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Cas No.: 53213-82-4
Sample solution is provided at 25 µL, 10mM.
3,3'-Dihexyloxacarbocyanine iodide is a carbocyanine dye which can be used to monitor changes in mitochondrial membrane potential.
Addition of cells to the cuvette containing 0.25 μM 3,3'-Dihexyloxacarbocyanine iodide (DiOCg(3)) leads to an increase in fluorescence, equilibration is rapid and is complete by 4 min. When f-met-leu-phe (10-7 M) is added to neutrophils preequilibrated with 3,3'-Dihexyloxacarbocyanine iodide, there is a short lag period of 5 seconds. The lag period is followed by a rapid loss of fluorescence. Examination of 3,3'-Dihexyloxacarbocyanine iodide loaded neutrophils using fluorescence microscopy demonstrates that in resting neutrophils the brightest fluorescence is associated with long thin organelles which corresponds to the distribution of mitochondria[1].
[1]. Korchak HM, et al. A carbocyanine dye, DiOC6(3), acts as a mitochondrial probe in human neutrophils. Biochem Biophys Res Commun. 1982 Oct 29;108(4):1495-501.
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