4,8-DiMeIQx |
Catalog No.GC78996
One-Click Copy Product Info
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4,8-DiMeIQx is an orally active mutagen, selective dopaminergic neurotoxicant, and DNA damaging agent.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 95896-78-9
Sample solution is provided at 25 µL, 10mM.
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In Vivo, 4,8-DiMeIQx (50 mg/kg; p.o.; single dose) forms DNA adducts in rat liver in vivo, with N2-(2'-deoxyguanosin-8-yl)-4,8-DiMeIQx as the major adduct representing ~60% of total adducts and having a relative adduct level of 3.54×10-5 at 72 hours post-single 50 mg/kg oral dose[3]. 4,8-DiMeIQx (0.5 mg per rat; p.o.; single dose) oral administration to male AGUS rats results in predominant urinary excretion of radioactivity within 24 hours, with BNF-induced intestinal enzyme activity increasing fecal excretion; major metabolites include hydroxylated and acetylated forms, with varying mutagenic potency relative to the parent compound[4].
In Vitro, 4,8-DiMeIQx (1 μg per assay) is mutagenic to Salmonella typhimurium TA98 with S9 mix, inducing 206,000 revertants per μg, and accounts for 9% of the total mutagenicity of bacteriological-grade beef extract[1]. 4,8-DiMeIQx (100 nM-5 μM; 24 h) is selectively neurotoxic to dopaminergic neurons in E17 rat primary midbrain cultures, with a threshold dose of 100 nM and significant dopaminergic neuron loss observed across all tested concentrations from 100 nM to 5 μM, while non-dopaminergic neurons remain unaffected[2]. 4,8-DiMeIQx (100 nM-5 μM; 24 h) does not elicit significant neurite length changes in either dopaminergic or non-dopaminergic neurons in E17 rat primary midbrain cultures at concentrations from 100 nM to 5 μM after 24 h of incubation[2].
References:
[1]. Takahashi M, et al. Identification and quantification of 2-amino-3,4,8-trimethylimidazo- [4,5-f]quinoxaline (4,8-DiMeIQx) in beef extract. Carcinogenesis. 1985 Oct;6(10):1537-9.
[2]. Cruz-Hernandez A, et al. Selective dopaminergic neurotoxicity of three heterocyclic amine subclasses in primary rat midbrain neurons. Neurotoxicology. 2018 Mar;65:68-84.
[3]. Frandsen H, et al. Formation of DNA adducts by the food mutagen 2-amino-3,4,8-trimethyl-3H-imidazo[4,5-f]quinoxaline (4,8-DiMeIQx) in vitro and in vivo. Identification of a N2-(2'-deoxyguanosin-8-yl)-4,8-DiMeIQx adduct. Carcinogenesis. 1994 Nov;15(11):2553-8.
[4]. Knize MG, et al. The metabolism of 4,8-DiMeIQx in conventional and germ-free rats. Carcinogenesis. 1989 Aug;10(8):1479-84.
| Cas No. | 95896-78-9 | SDF | |
| Formula | C12H13N5 | M.Wt | 227.27 |
| Solubility | Storage | Store at -20°C | |
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 4.4001 mL | 22.0003 mL | 44.0005 mL |
| 5 mM | 880 μL | 4.4001 mL | 8.8001 mL |
| 10 mM | 440 μL | 2.2 mL | 4.4001 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)