Antimycin A1 |
| Catalog No.GC42818 |
Antimycin A, an antibiotic produced by Streptomyces species that demonstrates antifungal, insecticidal, nematocidal, and piscicidal properties, is a mixture of Antimycins A1, A2, A3, and A4.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 642-15-9
Sample solution is provided at 25 µL, 10mM.
Antimycin A, an antibiotic produced by Streptomyces species that demonstrates antifungal, insecticidal, nematocidal, and piscicidal properties, is a mixture of Antimycins A1, A2, A3, and A4. [1] It blocks mitochondrial respiration and can deplete cellular levels of ATP via inhibition of complex III of the mitochondrial electron transport chain (ETC). Antimycin A prevents the transfer of electrons between the b-cytochromes and ubiquinone at the Q(inner) site of complex III. This results in the stabilization of the ubisemiquinone radical at the Q(outer) site of complex III, leading to increased production of superoxide. [2][3] Antimycin A is widely used in research to shunt electron flow through the ETC in order to study the chemical details of oxygen respiration. Additionally, antimycin A has been shown to inhibit Bcl-2 and Bcl-xL proteins, inducing apoptosis.[3][4][5]
Reference:
[1]. Seipke, R.F., and Hutchings, M.I. The regulation and biosynthesis of antimycins. Beilstein J.Org.Chem. 9, 2556-2563 (2013).
[2]. Muller, F., Crofts, A.R., and Kramer, D.M. Multiple Q-cycle bypass reactions at the Qo site of the cytochrome bc1 complex. Biochemistry 41(25), 7866-7874 (2002).
[3]. Muller, F.L., Roberts, A.G., Bowman, M.K., et al. Architecture of the Qo site of the cytochrome bc1 complex probed by superoxide production. Biochem. 42(21), 6493-6499 (2003).
[4]. Azmi, A.S., and Mohammad, R.M. Non-peptidic small molecule inhibitors against Bcl-2 for cancer therapy. J.Cell Physiol. 218(1), 13-21 (2009).
[5]. Marton, A., Mihalik, R., Bratincsak, A., et al. Apoptotic cell death induced by inhibitors of energy conservation--Bcl-2 inhibits apoptosis downstream of a fall of ATP level. Eur. J. Biochem. 250(2), 467-475 (1997).
| Cas No. | 642-15-9 | SDF | |
| Chemical Name | 2(or 3)-methyl-butanoic acid, (2R,3S,6S,7R,8R)-3-[[3-(formylamino)-2-hydroxybenzoyl]amino]-8-hexyl-2,6-dimethyl-4,9-dioxo-1,5-dioxonan-7-yl ester | ||
| Canonical SMILES | CC(C)CC(O[C@H]([C@H]1CCCCCC)[C@H](C)OC([C@@H](NC(C2=CC=CC(NC([H])=O)=C2O)=O)[C@@H](C)OC1=O)=O)=O | ||
| Formula | C28H40N2O9 | M.Wt | 548.6 |
| Solubility | Soluble in ethanol, methanol, DMSO, DMF | Storage | Store at -20°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.8228 mL | 9.1141 mL | 18.2282 mL |
| 5 mM | 0.3646 mL | 1.8228 mL | 3.6456 mL |
| 10 mM | 0.1823 mL | 0.9114 mL | 1.8228 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 35 reference(s) in Google Scholar.)
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