Br-DAPI |
Catalog No.GC68805
One-Click Copy Product Info
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Br-DAPI is a marker dye in the DAPI series. DAPI is a fluorescent dye that strongly binds to DNA. DAPI binds to the A/T base pairs in the minor groove of double-stranded DNA (one DAPI molecule can occupy three base pairs), resulting in an approximately 20-fold increase in fluorescence intensity. By observing fluorescence intensity, DNA can be quantified. DAPI can also penetrate intact cell membranes and is commonly used for staining live and fixed cells. Storage: Avoid light exposure.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 2387906-44-5
Sample solution is provided at 25 µL, 10mM.
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| Cas No. | 2387906-44-5 | SDF | |
| Formula | C16H14BrN5 | M.Wt | 356.22 |
| Solubility | DMSO : 100 mg/mL (280.73 mM; Need ultrasonic) | Storage | 4°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.8073 mL | 14.0363 mL | 28.0725 mL |
| 5 mM | 561.5 μL | 2.8073 mL | 5.6145 mL |
| 10 mM | 280.7 μL | 1.4036 mL | 2.8073 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >95.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)