Ceramides Mixture |
Catalog No.GC39484
One-Click Copy Product Info
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Ceramides Mixture is a main lipid component of the permeability barrier in the epidermis, where it plays a crucial role in maintaining skin integrity and barrier function.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 100403-19-8
Sample solution is provided at 25 µL, 10mM.
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Ceramides Mixture is a main lipid component of the permeability barrier in the epidermis, where it plays a crucial role in maintaining skin integrity and barrier function. Ceramides Mixture is used mainly as a moisturizer in various cosmetic products. The concentration of Ceramides Mixture in healthy skin was found to be approximately 46-53% of total protein-bound lipids, whereas in affected areas of atopic dermatitis, this percentage decreased to 23-28%[1].
In vitro, Ceramides Mixture was grafted onto hyaluronic acid (HA) for local drug delivery in the skin. The HA-Ceramides Mixture derivatives (10-1000μg/mL) showed no obvious cytotoxicity to NIH-3T3 cells , and the cell survival rate was similar to that of the untreated control group. The THP-1 cell line was differentiated into macrophages, and the inhibitory effect of HA-Ceramides Mixture on LPS-induced IL-6 production was detected by ELISA. Macrophages differentiated from THP-1 monocytic human cell line co-incubated with LPS (1μg/mL) and HA-Ceramides Mixture derivatives (250 and 100μg/mL, respectively). HA-Ceramides Mixture exhibited a statistically significant inhibition effect on IL-6 production and LPS-induced inflammatory response. The highest anti-inflammatory effect was observed for the 250μg/mL concentration[2]. Ceramides Mixture are extracted from Korean traditional plants including P. ginseng, C. sinensis (a green tea plant), G. max seoritae (a Korean native soybean variety), G. max napjakong (a Korean native soybean variety), and C. japonica. Normal human epidermal keratinocytes (NHEKs) treated with above-mentioned Ceramides Mixture (0-4μg/mL or 0-10μg/mL) for 24h increased the expression of epidermal differentiation-related genes (such as FLG, CASP14 and INV). Ceramides Mixture significantly accelerated the recovery rate of the damaged skin barrier and enhanced the adhesion of the stratum corneum, especially C. sinensis Ceramides Mixture[3].
References:
[1] Macheleidt O, Kaiser H W, Sandhoff K. Deficiency of epidermal protein-bound omega-hydroxyceramides in atopic dermatitis. J Invest Dermatol. 2002 Jul;119(1):166-73.
[2] Juhaščik M, Štarmanová K, Brandejsová M, et al. Synthesis and self-assembling of hyaluronan grafted with ceramide NP for topical drug delivery. Carbohydr Polym. 2023 Dec 1:321:121283.
[3] Choi H K, Hwang K H, Hong Y D, et al. Ceramide NPs derived from natural oils of Korean traditional plants enhance skin barrier functions and stimulate expressions of genes for epidermal homeostasis. J Cosmet Dermatol. 2022 Oct;21(10):4931-4941.
| Cell experiment [1]: | |
Cell lines | Normal human epidermal keratinocytes (NHEKs) |
Preparation Method | Ceramides Mixture were extracted from P. ginseng, C. sinensis (a green tea plant), G. max seoritae (a Korean native soybean variety), G. max napjakong (a Korean native soybean variety), and C. japonica, and purified by supercritical fluid extraction. The Ceramides Mixture derived from each oil were named P. ginseng Ceramides Mixture, C. sinensis Ceramides Mixture, G. max seoritae Ceramides Mixture, G. max napjakong Ceramides Mixture, and C. japonica Ceramides Mixture. NHEKs were treated with various concentrations of the above-mentioned Ceramides Mixtures for 24 hours, after which mRNA was extracted. |
Reaction Conditions | 0-4μg/mL or 0-10μg/mL; 24h |
Applications | Ceramides Mixture significant increase in the expression of epidermal differentiation-related genes (such as FLG, CASP14 and INV) from keratinocytes. C. sinensis Ceramides Mixture performs exceptionally well in enhancing the skin barrier function and promoting epidermal homeostasis. |
References: | |
| Cas No. | 100403-19-8 | SDF | |
| Canonical SMILES | [Ceramides Mixture] | ||
| Formula | C24H47NO3 | M.Wt | 397.63 |
| Solubility | Methanol: 25 mg/mL | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.5149 mL | 12.5745 mL | 25.149 mL |
| 5 mM | 503 μL | 2.5149 mL | 5.0298 mL |
| 10 mM | 251.5 μL | 1.2575 mL | 2.5149 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 4 reference(s) in Google Scholar.)