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Coenzyme FO

Catalog No.GC60720

Coenzyme FO, a deazaflavin chromophore, acts as an important hydride acceptor/donor in the central methanogenic pathway.

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Coenzyme FO Chemical Structure

Cas No.: 37333-48-5

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Sample solution is provided at 25 µL, 10mM.

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Background

Coenzyme FO, a deazaflavin chromophore, acts as an important hydride acceptor/donor in the central methanogenic pathway[1][2].

The formation of Coenzyme FO is mediated by two separate radical SAM active sites, one each in the CofG and CofH enzymes or both in the FbiC enzyme. These two radical SAM domains constitute the functional domains of Fo synthase. F420 is biosynthesized in Methanocaldococcus jannaschii by the action of eight enzymes with the formation of the deazaflavin chromophore (Fo) as the remaining unsolved step[1].Coenzyme F420 is the central low-redox-potential electron carrier in methanogenic metabolism. Coenzyme F420 is reduced under hydrogen by the action of F420-dependent hydrogenase[3].Coenzyme F420 acts as a hydride transfer coenzyme for an F420-specific glucose-6-phosphate dehydrogenase (Fgd) in mycobacteria. Coenzyme F420 is found in all methanogenic and certain nonmethanogenic archaea, where it participates in energy metabolism, NADP reduction, oxygen detoxification, and sulfite reduction. By converting NO2 back to NO with F420H2, M. tuberculosis could decrease the effectiveness of antibacterial action of macrophages[4].

[1]. Mills DJ, et al. De novo modeling of the F(420)-reducing [NiFe]-hydrogenase from a methanogenic archaeon by cryo-electron microscopy. Elife. 2013;2:e00218. Published 2013 Mar 5. [2]. Philmus B, et al. Biosynthetic versatility and coordinated action of 5'-deoxyadenosyl radicals in deazaflavin biosynthesis. J Am Chem Soc. 2015;137(16):5406-5413. [3]. de Poorter LMI, et al. Hydrogen concentrations in methane-forming cells probed by the ratios of reduced and oxidized coenzyme F420. Microbiology. 2005;151(Pt 5):1697‐1705. [4]. Purwantini E, et al. Conversion of NO2 to NO by reduced coenzyme F420 protects mycobacteria from nitrosative damage. Proc Natl Acad Sci U S A. 2009;106(15):6333‐6338.

Chemical Properties

Cas No. 37333-48-5 SDF
Canonical SMILES O[C@H]([C@H](O)[C@H](O)CO)CN(C1=CC(O)=CC=C1C=C2C(N3)=O)C2=NC3=O
Formula C16H17N3O7 M.Wt 363.32
Solubility DMSO: 5 mg/mL (13.76 mM) Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 2.7524 mL 13.762 mL 27.5239 mL
5 mM 0.5505 mL 2.7524 mL 5.5048 mL
10 mM 0.2752 mL 1.3762 mL 2.7524 mL
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Average Rating: 5 ★★★★★ (Based on Reviews and 37 reference(s) in Google Scholar.)

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