Dnp-PLGMWSR (trifluoroacetate salt) (Synonyms: Dnp-Pro-Leu-Gly-Met-Trp-Ser-Arg-OH, Matrix Metalloproteinase-2/Matrix Metalloproteinase-9 Fluorogenic Substrate I, MMP-2/MMP-9 Fluorogenic Substrate I) |
Catalog No.GC43550
One-Click Copy Product Info
|
Dnp-PLGMWSR (trifluoroacetate salt) is a fluorogenic heptapeptide substrates for 72kDa fibroblast gelatinase and 92kDa neutrophil gelatinase.
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
Dnp-PLGMWSR (trifluoroacetate salt) is a fluorogenic heptapeptide substrates for 72kDa fibroblast gelatinase and 92kDa neutrophil gelatinase [1]. Dnp-PLGMWSR is widely used as a fluorescent substrate for matrix metalloproteinase-9 (MMP-9) to determine the regulatory effect of Tofacitinib and CD147 on the expression of MMP-9 [2-3]. Dnp-PLGMWSR is generally empolyed to detect the hydrolytic activity of MMP-2/9 proteins for assessing the invasive effects of Jurkat-R and Sup-T1-R cells [4].
References:
[1] Netzel-Arnett S, Mallya S K, Nagase H, et al. Continuously recording fluorescent assays optimized for five human matrix metalloproteinases[J]. Analytical biochemistry, 1991, 195(1): 86-92.
[2] Rahat M M, Sabtan H, Simanovich E, et al. Soluble CD147 regulates endostatin via its effects on the activities of MMP-9 and secreted proteasome 20S[J]. Frontiers in Immunology, 2024, 15: 1319939.
[3] Zisman D, Sabtan H, Rahat M M, et al. Tofacitinib regulates endostatin via effects on CD147 and cathepsin S[J]. International Journal of Molecular Sciences, 2024, 25(13): 7267.
[4] Lin C, Xie Y, Huang W, et al. 5-Aza-dC promotes T-cell acute lymphoblastic leukemia cell invasion via downregulation of DNMT1 and upregulation of MMP-2 and MMP-9[J]. Experimental Hematology, 2022, 114: 43-53. e2.
| Cas No. | SDF | ||
| Synonyms | Dnp-Pro-Leu-Gly-Met-Trp-Ser-Arg-OH, Matrix Metalloproteinase-2/Matrix Metalloproteinase-9 Fluorogenic Substrate I, MMP-2/MMP-9 Fluorogenic Substrate I | ||
| Canonical SMILES | O=C(N[C@@H](CO)C(N[C@H](C(O)=O)CCCNC(N)=N)=O)[C@@H](NC([C@H](CCSC)NC(CNC([C@H](CC(C)C)NC([C@H]1N(C2=CC=C([N+]([O-])=O)C=C2[N+]([O-])=O)CCC1)=O)=O)=O)=O)CC3=CNC4=C3C=CC=C4.FC(F)(C(O)=O)F | ||
| Formula | C44H61N13O13S•XCF3COOH | M.Wt | 1012.1 |
| Solubility | Formic Acid: 1 mg/ml | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 988 μL | 4.9402 mL | 9.8804 mL |
| 5 mM | 197.6 μL | 988 μL | 1.9761 mL |
| 10 mM | 98.8 μL | 494 μL | 988 μL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >95.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 11 reference(s) in Google Scholar.)