Ercalcidiol (Synonyms: Ercalcidiol, 25Hydroxyergocalciferol, 25(OH)-Vitamin D2) |
Catalog No.GC13804
One-Click Copy Product Info
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Ercalcidiol is a metabolite of vitamin D2 that reflects the nutritional status of vitamin D in the body.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 21343-40-8
Sample solution is provided at 25 µL, 10mM.
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Ercalcidiol is a metabolite of vitamin D2 that reflects the nutritional status of vitamin D in the body[1]. Compared to vitamin D3 metabolites, Ercalcidiol has a shorter half-life and exhibits lower binding affinity to vitamin D-binding protein. Due to its relatively weaker potency, higher doses are required when used for therapeutic purposes[2]. Ercalcidiol is commonly employed for monitoring the efficacy of vitamin D2 treatment and managing disorders of calcium and phosphate homeostasis[3,4].
In vitro, Ercalcidiol (292.5nM) was incubated as a substrate in a renal homogenate system from rachitic chickens for 2h, where it was successfully metabolized by 1α-hydroxylase to produce 1α,25-(OH)2D2[5].
References:
[1] TUGCU G, CHAREHSAZ M, AYDIN A. Toxicological evaluation of ergocalciferol, cholecalciferol, and their metabolites by a category approach[J]. Drug and Chemical Toxicology, 2021, 44(6): 661-667.
[2] NEWMAN M S, BRANDON T R, GROVES M N, et al. A liquid chromatography/tandem mass spectrometry method for determination of 25-hydroxy vitamin D2 and 25-hydroxy vitamin D3 in dried blood spots: a potential adjunct to diabetes and cardiometabolic risk screening[J]. Journal of Diabetes Science and Technology, 2009, 3(1): 156-162.
[3] ADAMS J S, SHIEH A, BISHOP C W. Calcifediol as a therapeutic[M]//Feldman and Pike's Vitamin D. 5th ed. Cambridge: Academic Press, 2024: 457-474.
[4] JIN Z, BERTHOLF R L, YI X. Advances and challenges in the measurement of 1, 25-dihydroxyvitamin D: A comprehensive review[J]. Critical Reviews in Clinical Laboratory Sciences, 2023, 60(7): 535-548.
[5] HUGHES M R, BAYLINK D J, JONES P G, et al. Radioligand receptor assay for 25-hydroxyvitamin D2/D3 and 1 alpha, 25-dihydroxyvitamin D2/D3[J]. The Journal of Clinical Investigation, 1976, 58(1): 61-70.
| Cas No. | 21343-40-8 | SDF | |
| Synonyms | Ercalcidiol, 25Hydroxyergocalciferol, 25(OH)-Vitamin D2 | ||
| Chemical Name | (1S,3Z)-3-[(2E)-2-[(1R,3aS,7aR)-1-[(E,2R,5S)-6-hydroxy-5,6-dimethylhept-3-en-2-yl]-7a-methyl-2,3,3a,5,6,7-hexahydro-1H-inden-4-ylidene]ethylidene]-4-methylidenecyclohexan-1-ol | ||
| Canonical SMILES | CC(C=CC(C)C(C)(C)O)C1CCC2C1(CCCC2=CC=C3CC(CCC3=C)O)C | ||
| Formula | C28H44O2 | M.Wt | 412.66 |
| Solubility | DMF: 20 mg/ml,DMSO: 20 mg/ml,Ethanol: 20 mg/ml,Ethanol:PBS (pH 7.2)(1:2): 0.3 mg/ml | Storage | Store at -20°C,unstable in solution, ready to use. |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.4233 mL | 12.1165 mL | 24.233 mL |
| 5 mM | 484.7 μL | 2.4233 mL | 4.8466 mL |
| 10 mM | 242.3 μL | 1.2117 mL | 2.4233 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)