FM-red (Synonyms: PSH-red) |
Catalog No.GC61588
One-Click Copy Product Info
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FM-red (PSH-red) is a red-emitting and environment-sensitive probe for selectively detecting and labeling protein thiols.
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
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FM-red (PSH-red) is a red-emitting and environment-sensitive probe for selectively detecting and labeling protein thiols. FM-red can be used to image protein sulfhydryl groups in live cells and in vivo. FM-red also could be used to measure of the redox states of thioredoxin (Trx)[1].
FM-red (10 μM; 30 min) is suitable for imaging protein thiols in HeLa cells[1].FM-red (20 μM; 48 h) has no significant cytotoxicity in HeLa cells[1].FM-red possesses a long emission wavelength (~655 nm) and fast response (~10 min) by binding to protein thiols[1].FM-red exhibits time- and concentration-dependent fluorescence response to bovine serum albumin (BSA)[1].
FM-red (10 μM; 30 min) could be used to image protein thiols in zebrafishes[1].
[1]. Hu G, et, al. Depletion of protein thiols and the accumulation of oxidized thioredoxin in Parkinsonism disclosed by a red-emitting and environment-sensitive probe. J Mater Chem B. 2019 Apr 28;7(16):2696-2702.
| Cas No. | SDF | ||
| Synonyms | PSH-red | ||
| Canonical SMILES | CN(C1=CC=C(C2=[O+]C3=C(C=CC(N(CC)CC)=C3)C(C4=C(C=CC=C4)C(N5CCC(CC5)NC(CCCN6C(C=CC6=O)=O)=O)=O)=C2)C=C1)C.O=Cl(=O)([O-])=O | ||
| Formula | C41H46ClN5O9 | M.Wt | 788.29 |
| Solubility | DMSO : 50 mg/mL (63.43 mM; Need ultrasonic) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.2686 mL | 6.3428 mL | 12.6857 mL |
| 5 mM | 253.7 μL | 1.2686 mL | 2.5371 mL |
| 10 mM | 126.9 μL | 634.3 μL | 1.2686 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 20 reference(s) in Google Scholar.)