Nesfatin-1 (30-59) (mouse, rat) |
Catalog No.GA23241
One-Click Copy Product Info
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Nesfatin-1 (30-59) (mouse, rat) is the active core fragment of nesfatin-1 which regulates food intakes.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1872441-22-9
Sample solution is provided at 25 µL, 10mM.
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Nesfatin-1 (30-59) (mouse, rat) is the active core fragment of nesfatin-1 which regulates food intakes [1]. Nesfatin-1 (30-59) can upregulate the mRNA expression of proopiomelanocortin and amphetamine-regulated transcript, and act via an oxytocin-POMC-α-MSH/melanocortin receptor 3/4 pathway to inhibit food intake in rats [2]. Nesfatin-1 (30-59) has been widely used to regulate anxiety and depression-like behavior as well as anhedonia in rats under normal weight conditions [3].
In vivo, Nesfatin-1 (30-59) treatment via a single intracerebroventricular injection at a dose of 0.9nmol/rat (5μl) reduced the cumulative food intake of diet-induced obese rats within 20 hours[4].
References:
[1] Tekin O, Sevinç M M, Albayrak Ö, et al. Prognostic effect of Nesfatin-1 on the diagnosis and staging of acute cholecystitis[J]. Turkish Journal of Trauma & Emergency Surgery, 2022, 28(11): 1583.
[2] Prinz P, Teuffel P, Lembke V, et al. Nesfatin-130− 59 Injected Intracerebroventricularly Differentially Affects Food Intake Microstructure in Rats Under Normal Weight and Diet-Induced Obese Conditions[J]. Frontiers in neuroscience, 2015, 9: 422.
[3] Kühne S G, Schalla M A, Friedrich T, et al. Nesfatin-130-59 injected intracerebroventricularly increases anxiety, depression-like behavior, and anhedonia in normal weight rats[J]. Nutrients, 2018, 10(12): 1889.
[4] Prinz P, Teuffel P, Lembke V, et al. Nesfatin-130− 59 Injected Intracerebroventricularly Differentially Affects Food Intake Microstructure in Rats Under Normal Weight and Diet-Induced Obese Conditions[J]. Frontiers in neuroscience, 2015, 9: 422.
| Animal experiment [1]: | |
Animal models | Male Sprague-Dawley rats |
Preparation Method | Male Wistar rats weighing 280-350g were first group housed (4 rats/group) under controlled illumination (06.00-18.00h) and temperature (21-23°C). For the induction of diet-induced obesity (DIO), rats were fed a high fat diet (45% calories from fat, 35% from carbohydrates, and 20% from protein, 4.7kcal/g diet) for a period of 10 weeks. Nesfatin-1 (30-59) (0.9nmol/rat) or vehicle (5μl ddH2O) were injected i.c.v. directly at the beginning of the dark phase and the food intake microstructure was assessed within 24h. |
Dosage form | 0.9nmol (5μl) for once; i.c.v. |
Applications | Nesfatin-1 (30-59) treatment decreased meal frequency and reduced the food intake of DIO rats. |
References: | |
| Cas No. | 1872441-22-9 | SDF | |
| Formula | C167H260N40O54 | M.Wt | 3692.14 |
| Solubility | Soluble in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 270.8 μL | 1.3542 mL | 2.7085 mL |
| 5 mM | 54.2 μL | 270.8 μL | 541.7 μL |
| 10 mM | 27.1 μL | 135.4 μL | 270.8 μL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 1 reference(s) in Google Scholar.)