25-Hydroxy VD2-D6 (Synonyms: Ercalcidiol-d6, 25-Hydroxyergocalciferol-d6, 25(OH)-Vitamin D2-d6) |
Catalog No.GC35082
One-Click Copy Product Info
|
25-Hydroxy VD2-D6 is used as an internal standard for the quantification of 25-Hydroxy VD2 (Ercalcidiol, GC13804) by GC- or LC-MS. 25-Hydroxy VD2 is a metabolite of vitamin D2 that reflects the nutritional status of vitamin D in the body.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1262843-46-8
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
25-Hydroxy VD2-D6 is used as an internal standard for the quantification of 25-Hydroxy VD2 (Ercalcidiol, GC13804) by GC- or LC-MS. 25-Hydroxy VD2 is a metabolite of vitamin D2 that reflects the nutritional status of vitamin D in the body.[1]. 25-Hydroxy VD2-D6 can be used as a tracer. In vivo, 25-Hydroxy VD2 is formed from vitamin D2 by the cytochrome P450 (CYP) isoforms CYP2R1 and CYP27A1, and is bound to vitamin D binding protein (DBP) and albumin in the liver, then 25-Hydroxy VD2 is secreted into the bloodstream is transported to the kidney [2]. The serum level of 25-Hydroxy VD2 have been used as a marker to assess the vitamin D status [3].
References:
[1]. Tugcu, Gulcin, Mohammad Charehsaz, and Ahmet Aydın. "Toxicological evaluation of ergocalciferol, cholecalciferol, and their metabolites by a category approach." Drug and Chemical Toxicology 44.6 (2021): 661-667.
[2]. Bikle, D. "Vitamin D: production, metabolism, and mechanisms of action. MDText. com." Inc.: South Dartmouth, MA, USA (2000).
[3]. Saenger, Amy K., et al. "Quantification of serum 25-hydroxyvitamin D2 and D3using HPLC–Tandem Mass Spectrometry and examination of reference intervals for diagnosis of vitamin D deficiency." American journal of clinical pathology 125.6 (2006): 914-920.
| Cas No. | 1262843-46-8 | SDF | |
| Synonyms | Ercalcidiol-d6, 25-Hydroxyergocalciferol-d6, 25(OH)-Vitamin D2-d6 | ||
| Canonical SMILES | O[C@@H](CCC/1=C)CC1=C\C=C2[C@@](CC[C@@H]3[C@@H](/C=C/[C@H](C)C(C([2H])([2H])[2H])(C([2H])([2H])[2H])O)C)([H])[C@]3(C)CCC\2 | ||
| Formula | C28H38D6O2 | M.Wt | 418.68 |
| Solubility | DMSO : 100 mg/mL (238.85 mM; Need ultrasonic) | Storage | Store at -20°C, protect from light, stored under nitrogen |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.3885 mL | 11.9423 mL | 23.8846 mL |
| 5 mM | 477.7 μL | 2.3885 mL | 4.7769 mL |
| 10 mM | 238.8 μL | 1.1942 mL | 2.3885 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 26 reference(s) in Google Scholar.)