Luciferase |
Catalog No.GC36492
One-Click Copy Product Info
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Luciferase is an enzyme capable of catalyzing bioluminescent reactions.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 9014-00-0
Sample solution is provided at 25 µL, 10mM.
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Luciferase is an enzyme capable of catalyzing bioluminescent reactions. Luciferase catalyzes the oxidative decarboxylation of the substrate luciferin to convert chemical energy into light energy, and it can be used to reflect the expression level of a target gene by being coupled with that gene. Luciferase can be used in research related to gene expression regulation and in vivo imaging[1-4].
References:
[1] Schramm S, Al-Handawi MB, Karothu DP, et al. Mechanically Assisted Bioluminescence with Natural Luciferase. Angew Chem Int Ed Engl. 2020 Sep 14;59(38):16485-16489.
[2] Tu SC. Activity coupling and complex formation between bacterial luciferase and flavin reductases. Photochem Photobiol Sci. 2008 Feb;7(2):183-8.
[3] Dupont S. Luciferase Reporter Assays to Determine YAP/TAZ Activity in Mammalian Cells. Methods Mol Biol. 2019;1893:121-135.
[4] Yang T, Yuan Y, Ma F. Luciferase Reporter Systems in Investigating Interferon Antiviral Innate Immunity. Methods Mol Biol. 2025;2854:127-141.
Establishment of a Sustained Bioluminescence Imaging Detection Method Using Luc@CaP Nanoparticles Based on Luciferase[1]
This protocol is adapted from research data and provided for reference only. Adjustments may be necessary based on specific experimental requirements.
1. Reagent Preparation: Prepare a Luciferase solution at a concentration of 400μg/mL; use calcium phosphate as the mineralization carrier to prepare Luc@CaP nanoparticles (Luc@CaP NPs) via biomimetic mineralization, with an optimal mineralization time of 4 hours; synthesize and prepare a working solution of the probe Cys(SEt)-Lys-CBT (CKCBT) at a concentration of 300μM or amino-luciferin (Am-luciferin) at 300μM; prepare a detection buffer containing 1mM ATP and 10mM Mg²⁺.
2. In vitro Cellular Level Detection:
(1) Culture the cell line and seed the cells in a 96-well plate (approximately 2×10⁶ cells per well).
(2) Add 400μg/mL of Luc@CaP NPs to the cells and incubate for 8 hours to achieve cellular internalization and uptake.
(3) Aspirate the excess reagents and add 300μM of CKCBT or Am-luciferin probe working solution to the wells.
(4) Use a live imaging system to perform real-time bioluminescence imaging (BLI) at specific time points (0, 1, 2, 4, 6, 7 hours, etc.), and record and analyze the dynamic changes in luminescence intensity.
3. In vivo Animal Level Detection
(1) Establish an orthotopic cancer model by injecting 5×10⁶ cancer cells into BALB/c mice.
(2) When the tumor diameter reaches about 7-8mm, administer 4.0mg/kg of Luc@CaP NPs via intratumoral (i.t.) injection.
(3) Twenty minutes after administration, inject 200μmol/kg of CKCBT or 50μmol/kg of Am-luciferin via intraperitoneal (i.p.) injection.
(4) Use a small animal imaging system to continuously record the bioluminescent signals at the tumor site over a time window of up to 120 minutes.
Precautions:
(1) Animal experiments must comply with animal welfare guidelines.
(2) For your safety and health, please wear a lab coat and disposable gloves during operation.
References:
[1] Hong Y, Yang Y, Wang C, et al. Luciferase-Loaded Calcium Phosphate Nanoparticles for Persistent Bioluminescence Imaging of Orthotopic Breast Tumors. Anal Chem. 2024 Sep 10;96(36):14320-14325.
| Cas No. | 9014-00-0 | SDF | |
| Formula | M.Wt | ||
| Solubility | Water: 50 mg/mL | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 5 reference(s) in Google Scholar.)