Phytohemagglutinin |
Catalog No.GC36904
One-Click Copy Product Info
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Phytohemagglutinin is a plant-derived lectin protein.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 9008-97-3
Sample solution is provided at 25 µL, 10mM.
Phytohemagglutinin is a plant-derived lectin protein [1]. Phytohemagglutinin binds to the surface glycoproteins of T lymphocytes, activating and inducing their proliferation [2]. Phytohemagglutinin is often used cancer research [3].
In A549 cells, Phytohemagglutinin (20–160μg/mL; 48h) treatment caused a dose-dependent increase of cell growth inhibition and cytotoxicity on cells [4]. In Intestine-407, IEC-6, IEC-18 cells, Phytohemagglutinin (0.1µg/mL; 48-72h) significantly stimulated proliferation in all three cell lines [5]. In PC3 cells, Phytohemagglutinin (0.1–100μg/mL; 9d) has a significant dose-dependent toxic effect on prostate cancer cell proliferation [6].
In A20 cells subcutaneously tumor mice model, Phytohemagglutinin (1mg/mL; ip; 20d) treatment also inhibits tumor growth in mice [7]. In Krebs II cells subcutaneously tumor mice model, A diet containing the lectin Phytohemagglutinin (42mg; po; 8d) slows down the proliferation of Krebs II cell tumours in mice [8].
References:
[1]. Nagae M, Soga K, Morita-Matsumoto K, et al. Phytohemagglutinin from Phaseolus vulgaris (PHA-E) displays a novel glycan recognition mode using a common legume lectin fold[J]. Glycobiology, 2014, 24(4): 368-378.
[2]. Fujii T, Yamada S, Watanabe Y, et al. Induction of choline acetyltransferase mRNA in human mononuclear leukocytes stimulated by phytohemagglutinin, a T-cell activator[J]. Journal of neuroimmunology, 1998, 82(1): 101-107.
[3]. Gulden G, Sert B, Teymur T, et al. CAR-T cells with phytohemagglutinin (PHA) provide anti-cancer capacity with better proliferation, rejuvenated effector memory, and reduced exhausted t cell frequencies[J]. Vaccines, 2023, 11(2): 313.
[4]. Kuo W T, Ho Y J, Kuo S M, et al. Induction of the mitochondria apoptosis pathway by phytohemagglutinin erythroagglutinating in human lung cancer cells[J]. Annals of surgical oncology, 2011, 18(3): 848-856.
[5]. Otte J M, Chen C, Brunke G, et al. Mechanisms of lectin (phytohemagglutinin)-induced growth in small intestinal epithelial cells[J]. Digestion, 2001, 64(3): 169-178.
[6]. Camby I, Janssen T, De Decker R, et al. Lectin-induced alterations on the proliferation of three human prostatic cancer cell lines[J]. In Vitro Cellular & Developmental Biology-Animal, 1996, 32(10): 633-639.
[7]. D'Costa S S, Hurwitz J L. Phytohemagglutinin inhibits lymphoid tumor growth in vitro and in vivo[J]. Leukemia & lymphoma, 2003, 44(10): 1785-1791.
[8]. Pryme I F, Pusztai A J, Bardocz S. A diet containing the lectin phytohaemagglutinin (PHA) slows down the proliferation of Krebs II cell tumours in mice[J]. Cancer letters, 1994, 76(2-3): 133-137.
| Cell experiment [1]: | |
Cell lines | A549 cells |
Preparation Method | A549 cells were seeded at 10,000 cells per well in 24-well plates and incubated for 24h. The medium was then replaced with serum-free medium, Phytohemagglutinin (20–160μg/mL), and co-cultured for 48h. After treatment with Phytohemagglutinin, the medium was then replaced with 0.5mg/mL MTT medium and incubated for 4h. |
Reaction Conditions | 20–160μg/mL; 48h |
Applications | Phytohemagglutinin treatment caused a dose-dependent increase of cell growth inhibition and cytotoxicity on A549 cells. |
| Animal experiment [2]: | |
Animal models | A20 cells subcutaneously tumor mice model |
Preparation Method | A20 cells were injected subcutaneously into CBl7-SCID mice on day 0. On days 1 and 2, mice were injected intraperitoneally (i.p.) with 100μL of 1mg/mL of Phytohemagglutinin and/or 12mg/kg 5-FU. On days 3 and 4, the 5-FU treated mice received additional i.p. injections with 12mg/kg 5-FU. |
Dosage form | 1mg/mL; ip; 20d |
Applications | Phytohemagglutinin treatment also inhibits tumor growth in mice. |
References: | |
| Cas No. | 9008-97-3 | SDF | |
| Canonical SMILES | [Phytohemagglutinin] | ||
| Formula | M.Wt | ||
| Solubility | Water : 50 mg/mL | Storage | 4°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
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3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
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Average Rating: 5 (Based on Reviews and 34 reference(s) in Google Scholar.)