GPDA (GPN) |
Catalog No.GC30321
One-Click Copy Product Info
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GPDA (GPN) is the substrate for detecting glycylproline dipeptidyl aminopeptidase.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 65096-46-0
Sample solution is provided at 25 µL, 10mM.
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GPDA (GPN) is the substrate for detecting glycylproline dipeptidyl aminopeptidase. GPDA (GPN) can be hydrolyzed by the corresponding enzyme, producing yellow p-nitroaniline. By monitoring the change in absorbance at 405nm, enzyme activity can be quantified[1].
References:
[1] Hino M, Fuyamada H, Hayakawa T, et al. X-Prolyl dipeptidyl-aminopeptidase activity, with X-proline p-nitroanilides as substrates, in normal and pathological human sera. Clin Chem. 1976 Aug;22(8):1256-61.
GPDA Enzyme Activity Assay
This protocol is adapted from research data and provided for reference only. Adjustments may be necessary based on specific experimental requirements.
(1) Dissolve GPDA in 157.5mM Tris-HCl buffer (pH=7.9) to prepare a high-concentration stock solution (5mM). Aliquot and store at -20°C. Dissolve glycylglycine in Tris-HCl buffer (157.5mM, pH=7.9) to a concentration of 62.6mM.
(2) Enzyme Sample Preparation: Use homogenates of human liver tissue, liver cancer tissue, or embryonic tissue, or serum samples. For tissue homogenization, add 0.25M sucrose solution at a 1:9 (w/v) ratio.
(3) GPDA Enzyme Activity Detection: Reaction system: 50μL enzyme sample + 1mL reaction solution (containing 1.7mM GPDA substrate and 62.6mM glycylglycine). Incubate at 37°C for 30 minutes, then add 5 mL of 1M acetate buffer (pH=4.2) to terminate the reaction. Use a spectrophotometer to detect the absorbance of released p-nitroaniline at 405nm.
Precautions:
(1) The substrate is light-sensitive. Store and handle it protected from light to slow degradation.
(2) Post-mortem changes in tissue samples may affect enzyme activity (especially the stability of GPDA in microsomes is poor). GPDA is recommended to use fresh surgical samples rather than autopsy samples.
(3) For your safety and health, please wear a lab coat and disposable gloves during operation.
References:
[1] Kojima J, Ueno Y, Kasugai H, et al. Glycylproline dipeptidyl aminopeptidase and gamma-glutamyl transpeptidase in human hepatic cancer and embryonal tissues. Clin Chim Acta. 1987 Aug 31;167(3):285-91.
| Cas No. | 65096-46-0 | SDF | |
| Canonical SMILES | O=C([C@H](CCC1)N1C(CN)=O)NC2=CC=C([N+]([O-])=O)C=C2.O=S(C3=CC=C(C)C=C3)(O)=O | ||
| Formula | C20H24N4O7S | M.Wt | 464.49 |
| Solubility | DMSO : ≥ 48 mg/mL (103.34 mM) | Storage | Store at -20°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.1529 mL | 10.7645 mL | 21.529 mL |
| 5 mM | 430.6 μL | 2.1529 mL | 4.3058 mL |
| 10 mM | 215.3 μL | 1.0764 mL | 2.1529 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 24 reference(s) in Google Scholar.)