KRIBB3 |
Catalog No.GC18909 |
KRIBB3 is an Hsp27 and microtubule inhibitor that inhibits migration and invasion of MDA-MB-231 cells in vitro in an Hsp27-dependent manner.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 129414-88-6
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
KRIBB3 is an Hsp27 and microtubule inhibitor that inhibits migration and invasion of MDA-MB-231 cells in vitro in an Hsp27-dependent manner.[1] [2] It inhibits protein kinase C (PKC) phosphorylation of Hsp27 but not phospholipase Cβ3, indicating KRIBB3 interaction with Hsp27 is preventing its phosphorylation. KRIBB3 (1-100 μM) inhibits growth of HCT116, HCT15, and MDA-MB-231 cells in an Hsp27-independent and concentration-dependent manner. It induces HCT116 cell cycle arrest in the G2/M phase via formation of the Mad2/p55CDC inhibitory complex and Bax activation of apoptosis.[2] KRIBB3 also inhibits microtubule polymerization in HCT116 cells. In vivo, KRIBB3 (50-100 mg/kg) reduces tumor volume in an HCT116 mouse xenograft model.
Reference:
[1]. Shin, K.D., Lee, M.-Y., Shin, D.-S., et al. Blocking tumor cell migration and invasion with biphenyl isoxazole derivative KRIBB3, a synthetic molecule that inhibits Hsp27 phosphorylation. J. Biol. Chem. 280(50), 41439-41448 (2005).
[2]. Shin, K.D., Yoon, Y.J., Kang, Y.-R., et al. KRIBB3, a novel microtubule inhibitor, induces mitotic arrest and apoptosis in human cancer cells. Biochem. Pharmacol. 75(2), 383-394 (2008).
Cas No. | 129414-88-6 | SDF | |
Chemical Name | 4-ethyl-5-methoxy-2-[4-(4-methoxyphenyl)-5-isoxazolyl]-phenol | ||
Canonical SMILES | OC1=CC(OC)=C(CC)C=C1C2=C(C3=CC=C(OC)C=C3)C=NO2 | ||
Formula | C19H19NO4 | M.Wt | 325.4 |
Solubility | 25mM in DMSO | Storage | Store at -20°C |
General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. |
Prepare stock solution | |||
1 mg | 5 mg | 10 mg | |
1 mM | 3.0731 mL | 15.3657 mL | 30.7314 mL |
5 mM | 0.6146 mL | 3.0731 mL | 6.1463 mL |
10 mM | 0.3073 mL | 1.5366 mL | 3.0731 mL |
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Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
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