mCAP (Synonyms: m7G(5')ppp(5')G Cap Analog, N7-Methyl-Guanosine-5'-Triphosphate-5'-Guanosine) |
Catalog No.GC12563
One-Click Copy Product Info
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m7G(5')ppp(5')G Cap Analog, used to forming Cap 0 structure
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
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mRNA of eucaryon and virus has a monomethylated cap structure, m7G(5')ppp(5')G or called mCAP, at the 5’-initiating terminal. The cap analog plays an important role in cellular processes, the initiation of protein synthesis, protecting RNA from exonuclease cleavage, uniquely recognition for recruiting protein factors for pre-mRNA splicing, polyadenylation and nuclear export. The cap could interact with different binding proteins, initiation factors and elements of ribosomes.
mCAP is the first cap analog for the molecular biology toolbox. MCAP inserts in the correct orientation to enhance translation with a 50% chance. The other 50% of molecules can’t be a substrate for efficient translation, reducing the specific activity of the transcript.
mCAP can be incorporated in a transcription by including a mixture of the cap analog and GTP (usually at a 4:1 ratio). Approximately 80% of synthesized mRNA will possess a 5’ cap, while the remaining 20% will possess a 5’ triphosphate.
References:
[1].Abbas YM1, Laudenbach BT2, et al, Structure of human IFIT1 with capped RNA reveals adaptable mRNA binding and mechanisms for sensing N1 and N2 ribose 2'-O methylations. Proc Natl Acad Sci U S A. 2017 Mar 14;114(11):E2106-E2115.
[2].Katibah GE1, Qin Y2, et al, Broad and adaptable RNA structure recognition by the human interferon-induced tetratricopeptide repeat protein IFIT5. Proc Natl Acad Sci U S A. 2014 Aug 19;111(33):12025-30.
[3]. Kuge H1, Brownlee GG, et al, Cap ribose methylation of c-mos mRNA stimulates translation and oocyte maturation in Xenopus laevis. Nucleic Acids Res. 1998 Jul 1;26(13):3208-14.
[4]. Ramanathan A1, Robb GB1, et al, mRNA capping: biological functions and applications. Nucleic Acids Res. 2016 Sep 19;44(16):7511-26.
[5]. Probst J1, Brechtel S, et al, Characterization of the ribonuclease activity on the skin surface. Genet Vaccines Ther. 2006 May 29;4:4.
[6]. Karikó K1, Buckstein M, et al, Suppression of RNA recognition by Toll-like receptors: the impact of nucleoside modification and the evolutionary origin of RNA. Immunity. 2005 Aug;23(2):165-75.
| Cas No. | SDF | ||
| Synonyms | m7G(5')ppp(5')G Cap Analog, N7-Methyl-Guanosine-5'-Triphosphate-5'-Guanosine | ||
| Canonical SMILES | OC1C(COP([O-])(OP([O-])(OP([O-])(OCC2OC(N3C=[N+](C)C4=C3N=C(N)NC4=O)C(O)C2O)=O)=O)=O)OC(N5C=NC6=C5N=C(N)NC6=O)C1O | ||
| Formula | C21H30N10O18P3 (free acid) | M.Wt | 803.4 g/mol (free acid) |
| Solubility | Storage | Store at -20°C or below | |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.2447 mL | 6.2235 mL | 12.4471 mL |
| 5 mM | 248.9 μL | 1.2447 mL | 2.4894 mL |
| 10 mM | 124.5 μL | 622.4 μL | 1.2447 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 1 reference(s) in Google Scholar.)