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N-Ethylmaleimide Catalog No.GC34682

A cysteine protease inhibitor

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10 mM * 1 mL in DMSO
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In stock

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Sample solution is provided at 25 µL, 10mM.

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Chemical Properties

Cas No. 128-53-0 SDF Download SDF
Synonyms NEM
Chemical Name N/A
Canonical SMILES O=C(C=C1)N(CC)C1=O
Formula C6H7NO2 M.Wt 125.13
Solubility DMSO : 50 mg/mL (399.58 mM; Need ultrasonic) H2O : 50 mg/mL (399.58 mM; Need ultrasonic), Ethanol : 12.5 mg/mL (99.90 mM; Need ultrasonic) Storage Store at 4°C, sealed storage, away from moisture and light
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
Shipping Condition Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
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**When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / CoA (available online).



N-Ethylmaleimide (NEM), a reagent that alkylates free sulfhydryl groups, is a cysteine protease inhibitor[1]. N-ethylmaleimide specific inhibits phosphate transport in mitochondria[2]. N-Ethylmaleimide is also a deubiquitinating enzyme inhibitor[3].

[1]. Wu KH, et al. Cys32 and His105 are the critical residues for the calcium-dependent cysteine proteolyticactivity of CvaB, an ATP-binding cassette transporter. J Biol Chem. 2004 Jan 9;279(2):901-9.
[2]. Hatase O , et al. Specific inhibition of phosphate transport in mitochondria by N-ethylmaleimide. Journal of Bioenergetics, 1973, 5(1):1-15.
[3]. Choo YS, et al. Detection of protein ubiquitination. J Vis Exp. 2009;(30):1293. Published 2009 Aug 19. doi:10.3791/1293