|N-Ethylmaleimide Catalog No.GC34682|
Sample solution is provided at 25 µL, 10mM.
GlpBio Products Cited In Reputable Papers
|Cas No.||128-53-0||SDF||Download SDF|
|Solubility||DMSO : 50 mg/mL (399.58 mM; Need ultrasonic) H2O : 50 mg/mL (399.58 mM; Need ultrasonic), Ethanol : 12.5 mg/mL (99.90 mM; Need ultrasonic)||Storage||Store at 4°C, sealed storage, away from moisture and light|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|
|Shipping Condition||Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
N-Ethylmaleimide (NEM), a reagent that alkylates free sulfhydryl groups, is a cysteine protease inhibitor. N-ethylmaleimide specific inhibits phosphate transport in mitochondria. N-Ethylmaleimide is also a deubiquitinating enzyme inhibitor.
. Wu KH, et al. Cys32 and His105 are the critical residues for the calcium-dependent cysteine proteolyticactivity of CvaB, an ATP-binding cassette transporter. J Biol Chem. 2004 Jan 9;279(2):901-9.
. Hatase O , et al. Specific inhibition of phosphate transport in mitochondria by N-ethylmaleimide. Journal of Bioenergetics, 1973, 5(1):1-15.
. Choo YS, et al. Detection of protein ubiquitination. J Vis Exp. 2009;(30):1293. Published 2009 Aug 19. doi:10.3791/1293