Naringinase |
Catalog No.GC61697
One-Click Copy Product Info
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Naringinase is a complex enzyme with dual activities of α-L-rhamnosidase and β-D-glucosidase.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 9068-31-9
Sample solution is provided at 25 µL, 10mM.
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Naringinase is a complex enzyme with dual activities of α-L-rhamnosidase and β-D-glucosidase. Naringinase can hydrolyze naringin. Naringinase enhances the bioavailability of flavonoids through flavonoid deglycosylation. Naringinase can be used for research on debittering citrus juice, flavonoid deglycosylation, and improving wine flavor[1-4].
In vitro, MFEN (8:1) prepared by co-incubating mulberry fruit extract with Naringinase was used to pretreat IgE-sensitized RBL-2H3 cells at 0-200µg/mL for 1 hour, followed by antigen stimulation for 4 hours. MFEN inhibited β-hexosaminidase and TNF-α release. MFEN reduced PGD2 and LTC4 production. MFEN suppressed phosphorylation of Syk, PLCγ1/2, PKCδ, LAT, ERK1/2, JNK, Gab2, PI3K, Akt, cPLA2, and 5-LO. MFEN suppressed COX-2 expression[5].
In vivo, Kunming mice received oral gavage of 1.00-10.0g/kg Naringinase once daily for 14 days. Naringinase did not cause scratching, jumping, respiratory distress, cyanosis, or paralysis in the mice. Naringinase did not alter fur color, eye color, agility, sensitivity, or defecation in the mice[6].
References:
[1] Bodakowska-Boczniewicz J, Garncarek Z. Use of Naringinase to Modify the Sensory Quality of Foods and Increase the Bioavailability of Flavonoids: A Systematic Review. Molecules. 2025 May 29;30(11):2376.
[2] Hallikeri NS, Yaraguppi DA, Gangadharappa BS, et al. Naringinase: A comprehensive review on its characteristics, production, and biotechnological applications. Enzyme Microb Technol. 2026 Apr;195:110808.
[3] Puri M. Updates on naringinase: structural and biotechnological aspects. Appl Microbiol Biotechnol. 2012 Jan;93(1):49-60.
[4] Salunkhe JD, Patil SV. Improved naringinase double screen plate assay: progress towards the perfect screening. Nat Prod Res. 2025 Feb;39(3):609-612.
[5] Yoo JM, Kim NY, Seo JM, et al. Inhibitory effects of mulberry fruit extract in combination with naringinase on the allergic response in IgE-activated RBL-2H3 cells. International Journal of Molecular Medicine. 2014;33(2):469-477.
[6] Ni H, Chen F, Cai H, et al. Characterization and preparation of Aspergillus niger naringinase for debittering citrus juice. Journal of Food Science. 2012;77(1):C2-C7.
| Cell experiment [1]: | |
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Cell lines |
RBL-2H3 cells (rat basophilic leukemia cell line) |
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Preparation Method |
RBL-2H3 cells were maintained in MEM supplemented with 5% FBS, 100U/ml penicillin and 100μg/ml streptomycin at 37°C, 5% CO2. Cells were sensitized with 1μg/ml DNP-IgE for 24h, then treated with Naringinase in combination with mulberry fruit extract (1:8; 0-200μg/mL) for 1h, challenged with 25ng/ml DNP-HSA for 4h; assays included WST-1 cytotoxicity, β-hexosaminidase release, TNF-α ELISA, PGD2/LTB4/LTC4 EIA, and immunoblot for phosphorylation of Syk/Lyn/Fyn/PLCγ1/2/PKCδ/LAT/ERK1/2/JNK/Gab2/PI3K/Akt/cPLA2/5-LO and COX-2 expression. |
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Reaction Conditions |
0-200μg/mL (Naringinase : mulberry fruit extract=1:8); 1h pretreatment + 4h antigen challenge |
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Applications |
Naringinase in combination with mulberry fruit extract (MFEN) inhibited β-hexosaminidase and TNF-α release, reduced PGD2 and LTC4 formation, suppressed phosphorylation of Syk/PLCγ1/2/PKCδ/LAT/ERK1/2/JNK/Gab2/PI3K/Akt/cPLA2/5-LO, and downregulated COX-2 expression in IgE-activated RBL-2H3 cells, with no cytotoxicity in the tested range. |
| Animal experiment [2]: | |
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Animal models |
Kunming strain mice |
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Preparation Method |
Mice were acclimated for 7d at 21 to 25°C, 40% to 70% humidity, fasted 5h, then orally gavaged once daily for 14 days with Naringinase powder dissolved in purified water at 1.00, 2.15, 4.64, or 10.0g/kg; general health, toxicity symptoms, mortality, body weight, and food intake were monitored 3 times daily during administration. |
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Dosage form |
1.00-10.0g/kg; p.o.; once daily for 14 days |
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Applications |
Naringinase powder administration caused no mortality, no toxicity symptoms (scratching, jumping, respiratory distress, cyanosis, paralysis), no abnormal fur/eye color or action, and no significant difference in body weight gain or food intake compared with control in Kunming mice. |
References: [1] Yoo JM, Kim NY, Seo JM, et al. Inhibitory effects of mulberry fruit extract in combination with naringinase on the allergic response in IgE-activated RBL-2H3 cells. International Journal of Molecular Medicine. 2014;33(2):469-477. [2] Ni H, Chen F, Cai H, et al. Characterization and preparation of Aspergillus niger naringinase for debittering citrus juice. Journal of Food Science. 2012;77(1):C2-C7. | |
| Cas No. | 9068-31-9 | SDF | |
| Canonical SMILES | [Naringinase] | ||
| Formula | M.Wt | ||
| Solubility | Water: 120 mg/mL | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 3 reference(s) in Google Scholar.)