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Oxy-16 (Synonyms: 20(R),22(R)-dihydroxy Cholesterol)

Catalog No.GC17713

Oxy-16 is an endogenous metabolic intermediate.

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Oxy-16 Chemical Structure

Cas No.: 596-94-1

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10mg
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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Background

Oxy-16 is an antagonist of hedgehog activity.

Naturally occurring oxysterols that are products of cholesterol oxidation can stimulate the hedgehog (Hh) signaling pathway related to cardiovascular disease and bone formation. Activation of Hh signaling modulates inflammatory responses to additional atherogenic factors including lesion-producing macrophages and enable osteoblast differentiation, which is dependent on the target cell.

In vitro: Oxysterols have been shown to be associated with immunosuppression, apoptosis, atherosclerosis, inflammation, and cholesterol turnover. Other studies aslo indicated that oxysterols were Hedgehog (Hh) signaling pathway activators and had potent osteoinductive properties. Oxy-16, an oxysterol, was identified as a cell permeable oxygenated derivative of cholesterol that might be the end product or intermediate of the cholesterol excretion pathways. Oxy-16 is currently used as a transport form for cholesterol across the blood brain barrier and membranes. The osteogenic differentiation caused by Oxy-16 was cinsidered to be mediated via a Wnt signaling-related, Dkk-1-inhibitable mechanism [1].

In vivo: Up to now, there is no animal in vivo data reported.

Clinical trial: So far, no clinical study has been conducted.

Reference:
[1] Amantea, C. M.,Kim, W.K.,Meliton, V., et al. Oxysterol-induced osteogenic differentiation of marrow stromal cells is regulated by Dkk-1 inhibitable and PI3-kinase mediated signaling. Journal of Cellular Biochemistry 105(2), 424-436 (2008).

Chemical Properties

Cas No. 596-94-1 SDF
Synonyms 20(R),22(R)-dihydroxy Cholesterol
Chemical Name cholest-5-ene-3b,20,22R-triol
Canonical SMILES O[C@H](C1)CC[C@@]2(C)C1=CC[C@]3([H])C2CC[C@@]4(C)[C@@]3([H])CC[C@]4([H])[C@@](C)(O)[C@H](O)CCC(C)C
Formula C27H46O3 M.Wt 418.7
Solubility ≤10mg/ml in ethanol;1.5mg/ml in DMSO;1.5mg/ml in dimethyl formamide Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 2.3883 mL 11.9417 mL 23.8834 mL
5 mM 0.4777 mL 2.3883 mL 4.7767 mL
10 mM 0.2388 mL 1.1942 mL 2.3883 mL
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Average Rating: 5 ★★★★★ (Based on Reviews and 13 reference(s) in Google Scholar.)

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