Phosphatase Inhibitor Cocktail I (100× in DMSO) |
| Catalog No.: GK10011 |
Phosphatase Inhibitor Cocktail I effectively inhibits alkaline phosphatases and serine/threonine protein phosphatases such as PP1 and PP2A.
Sample solution is provided at 25 µL, 10mM.
- View current batch:
Thaw at room temperature. Before assaying, add each solution at 1:100 (v/v) dilution to samples (such as cell lysates or tissue extracts). Mix the sample sufficiently after each time of addition.
Application Fields: Western Blot analysis, Co-IP, pull-down, IF, IHC, kinase assay and etc.
Phosphatase Inhibitor Cocktail I effectively inhibits alkaline phosphatases and serine/threonine protein phosphatases such as PP1 and PP2A.
Phosphorylation of proteins and lipids is a hallmark of the signaling or metabolic status of cells. A cocktail of three inhibitors that will inhibit alkaline phosphatases as well as serine/threonine protein phosphatases such as PP1 and PP2A. Each vial contains 1 ml phosphatase inhibitor cocktail solubilized in DMSO with the following components: Bromotetramisole oxalate (2.5 mM), Cantharidin (500 µM), and Microcystin-LR , Microcystis aeruginosa (500 nM).
Phosphatase Inhibitor Cocktail I has been optimized and tested for mammalian cell and tissue extracts. This cocktail is supplied as a ready-to-use solution in DMSO.
Components
| Ingredient | M.W. | 100x Conc. | Target |
| (-)-p-Bromotetramisole oxalate | 995.2 | 2.5 mM | Alkaline phosphatases |
| Cantharidin | 373.2 | 500 μM | Ser/Thr phosphatases |
| Microcystin LR, Microcystis aeruginosa | 196.2 | 500 nM | PP1 and PP2A |
Average Rating: 5 (Based on Reviews and 10 reference(s) in Google Scholar.)
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