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Phosphatase Inhibitor Cocktail I (100× in DMSO) Catalog No.GK10011

Phosphatase Inhibitor Cocktail I effectively inhibits alkaline phosphatases and serine/threonine protein phosphatases such as PP1 and PP2A.

Size Price Stock Qty
1 mL
$26.10
In stock
1 mL × 10
$184.50
In stock
1 mL× 50
$778.00
In stock
1 mL × 100
$1,165.00
In stock

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Sample solution is provided at 25 µL, 10mM.

Protocol

Thaw at room temperature. Before assaying, add each solution at 1:100 (v/v) dilution to samples (such as cell lysates or tissue extracts). Mix the sample sufficiently after each time of addition.

Application Fields: Western Blot analysis, Co-IP, pull-down, IF, IHC, kinase assay and etc.

Background

Phosphatase Inhibitor Cocktail I effectively inhibits alkaline phosphatases and serine/threonine protein phosphatases such as PP1 and PP2A.

Phosphorylation of proteins and lipids is a hallmark of the signaling or metabolic status of cells. A cocktail of three inhibitors that will inhibit alkaline phosphatases as well as serine/threonine protein phosphatases such as PP1 and PP2A. Each vial contains 1 ml phosphatase inhibitor cocktail solubilized in DMSO with the following components: Bromotetramisole oxalate (2.5 mM), Cantharidin (500 µM), and Microcystin-LR , Microcystis aeruginosa (500 nM).

Phosphatase Inhibitor Cocktail I has been optimized and tested for mammalian cell and tissue extracts. This cocktail is supplied as a ready-to-use solution in DMSO.

Components

Ingredient M.W. 100x Conc. Target
(-)-p-Bromotetramisole oxalate 995.2 2.5 mM Alkaline phosphatases
Cantharidin 373.2 500 μM Ser/Thr phosphatases
Microcystin LR, Microcystis aeruginosa 196.2 500 nM PP1 and PP2A