Pyocyanin
Catalog No.: GC13137Purity:>98.00%Synonyms: Sanasin,Sanazin,Pyocyanine
Pyocyanin is a toxic metabolite produced by the Gram-negative bacterium Pseudomonas aeruginosa.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 85-66-5
10mM (in 1mL DMSO)In Stock
$88.00
1
5mgIn Stock
$80.00
1
10mgIn Stock
$145.00
1
25mgIn Stock
$305.00
1
50mgIn Stock
$545.00
1
100mgIn Stock
$960.00
1
| Pack Size | Price | Availability | Quantity | Action |
|---|---|---|---|---|
| 10mM (in 1mL DMSO) | $88.00 | In Stock | 1 | |
| 5mg | $80.00 | In Stock | 1 | |
| 10mg | $145.00 | In Stock | 1 | |
| 25mg | $305.00 | In Stock | 1 | |
| 50mg | $545.00 | In Stock | 1 | |
| 100mg | $960.00 | In Stock | 1 |
Tel: (909) 407-4943 / (909) 689-7124Email: sales@glpbio.com
Citations
1 publicationsPublications from researchers using this productDescription
Pyocyanin is a toxic metabolite produced by the Gram-negative bacterium Pseudomonas aeruginosa[1]. Pyocyanin is a blue secondary metabolite that turns red when the pH is below 4.9 and possesses redox activity.[2]. Pyocyanin is commonly applied in research on bacterial pathogenesis, host immune responses, and cytotoxicity assessment in chronic infection models[3,4].
In vitro, treatment of human pulmonary mucoepidermoid carcinoma NCI-H292 cells with Pyocyanin (25μg/mL) for 24h induced an approximately 4-fold increase in the transcriptional level of the mucin synthesis gene MUC5AC[5]. Treatment of A549 cells with Pyocyanin (1, 5, 10μM) for 24h showed no effect on cell growth at 1μM, transient inhibition of proliferation at 5μM, and complete and sustained growth arrest at 10μM[6].
In vivo, Pyocyanin (25mg/kg/day; p.o.) treatment of mice was initiated on day 21 post-infection with Schistosoma mansoni (larval stage) and continued for 5 days. On day 49 post-infection, the total worm burden (23.83 ± 2.79) was significantly reduced compared with that of the control group (36.33 ± 3.98), with a reduction rate of 34.41%[7].
References:[1] Hall S, McDermott C, Anoopkumar-Dukie S, et al. Cellular effects of pyocyanin, a secreted virulence factor of Pseudomonas aeruginosa[J]. Toxins, 2016, 8(8): 236.
[2] Hassan H M, Fridovich I. Mechanism of the antibiotic action pyocyanine[J]. Journal of bacteriology, 1980, 141(1): 156-163.
[3] Jayaseelan S, Ramaswamy D, Dharmaraj S. Pyocyanin: production, applications, challenges and new insights[J]. World Journal of Microbiology and Biotechnology, 2014, 30(4): 1159-1168.
[4] Rada B, Leto T L. Pyocyanin effects on respiratory epithelium: relevance in Pseudomonas aeruginosa airway infections[J]. Trends in microbiology, 2013, 21(2): 73-81.
[5] Caldwell C C, Chen Y, Goetzmann H S, et al. Pseudomonas aeruginosa exotoxin pyocyanin causes cystic fibrosis airway pathogenesis[J]. The American journal of pathology, 2009, 175(6): 2473-2488.
[6] Muller M. Premature cellular senescence induced by pyocyanin, a redox-active Pseudomonas aeruginosa toxin[J]. Free Radical Biology and Medicine, 2006, 41(11): 1670-1677.
[7] Eltarahony M M, Younis S S, Abdel Salam S A, et al. An insight into pyocyanin: production, characterization, and evaluation of its in vitro antibacterial, antifungal, antibiofilm and in vivo anti-schistosomal potency[J]. BMC microbiology, 2025, 25(1): 532.
Protocol
| Cell experiment [1]: | |
|
Cell lines |
NCI-H292 cells |
|
Preparation Method |
NCI-H292 cells were treated with 25μg/mL Pyocyanin for 24h, then total cellular RNA was extracted and subjected to qRT-PCR analysis using specific primers for MUC5AC and GAPDH (as internal control) to assess MUC5AC mRNA expression levels. |
|
Reaction Conditions |
25μg/mL; 24h |
|
Applications |
Treatment with Pyocyanin induced an approximately 4-fold increase in the transcriptional level of the mucin synthesis gene MUC5AC. |
| Animal experiment [2]: | |
|
Animal models |
Swiss albino mice infected with S. mansoni |
|
Preparation Method |
Swiss albino mice infected with S. mansoni cercariae were orally given Pyocyanin (25mg/kg/day) for 5 days from day 21 post-infection. On day 49, adult worms were recovered by perfusion and counted to calculate worm reduction. |
|
Dosage form |
25mg/kg/day; 5 days; p.o. |
|
Applications |
Pyocyanin was used to treat mice for 5 days starting on day 21 of infection with S. mansoni. At day 49, the total worm burden (23.83 ± 2.79) was significantly lower than that of the control group (36.33 ± 3.98), with a reduction rate of 34.41%. |
| References: [1] Caldwell C C, Chen Y, Goetzmann H S, et al. Pseudomonas aeruginosa exotoxin pyocyanin causes cystic fibrosis airway pathogenesis[J]. The American journal of pathology, 2009, 175(6): 2473-2488. [2] Eltarahony M M, Younis S S, Abdel Salam S A, et al. An insight into pyocyanin: production, characterization, and evaluation of its in vitro antibacterial, antifungal, antibiofilm and in vivo anti-schistosomal potency[J]. BMC microbiology, 2025, 25(1): 532. | |
Product Documents
Purity:>98.00%Appearance:A solid
Biological Data
1 / 1
Chemical Properties
CAS No.
85-66-5
Synonyms
Sanasin,Sanazin,Pyocyanine
Chemical Name
5-methyl-1(5H)-phenazinone
SMILES
Cn1c2ccccc2nc2c(=O)cccc12
Molecular Formula
C13H10N2O
Molecular Weight
210.2
Solubility
5mg/ml in ethanol, or in DMSO; 2.5mg/ml in DMF
Storage
Store at -20°C
General tips
Select a suitable solvent according to the product solubility data. Once prepared, aliquot the stock solution to avoid repeated freeze-thaw cycles.
Use stock solutions within 6 months when stored at -80°C or within 1 month when stored at -20°C.
To improve solubility, warm the tube to 37°C and sonicate it for an appropriate period.
Use stock solutions within 6 months when stored at -80°C or within 1 month when stored at -20°C.
To improve solubility, warm the tube to 37°C and sonicate it for an appropriate period.
Shipping Condition
Evaluation samples are shipped with blue ice. Other available sizes are shipped at room temperature or with blue ice upon request.
CalculatorsMolarity / Dilution / Molecular Weight / Unit Conversion / In Vivo Formulation / Solubility
g/mol
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