Pyocyanin (Synonyms: Sanasin,Sanazin,Pyocyanine) |
Catalog No.GC13137
One-Click Copy Product Info
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Pyocyanin is a toxic metabolite produced by the Gram-negative bacterium Pseudomonas aeruginosa.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 85-66-5
Sample solution is provided at 25 µL, 10mM.
Pyocyanin is a toxic metabolite produced by the Gram-negative bacterium Pseudomonas aeruginosa[1]. Pyocyanin is a blue secondary metabolite that turns red when the pH is below 4.9 and possesses redox activity.[2]. Pyocyanin is commonly applied in research on bacterial pathogenesis, host immune responses, and cytotoxicity assessment in chronic infection models[3,4].
In vitro, treatment of human pulmonary mucoepidermoid carcinoma NCI-H292 cells with Pyocyanin (25μg/mL) for 24h induced an approximately 4-fold increase in the transcriptional level of the mucin synthesis gene MUC5AC[5]. Treatment of A549 cells with Pyocyanin (1, 5, 10μM) for 24h showed no effect on cell growth at 1μM, transient inhibition of proliferation at 5μM, and complete and sustained growth arrest at 10μM[6].
In vivo, Pyocyanin (25mg/kg/day; p.o.) treatment of mice was initiated on day 21 post-infection with Schistosoma mansoni (larval stage) and continued for 5 days. On day 49 post-infection, the total worm burden (23.83 ± 2.79) was significantly reduced compared with that of the control group (36.33 ± 3.98), with a reduction rate of 34.41%[7].
References:[1] Hall S, McDermott C, Anoopkumar-Dukie S, et al. Cellular effects of pyocyanin, a secreted virulence factor of Pseudomonas aeruginosa[J]. Toxins, 2016, 8(8): 236.
[2] Hassan H M, Fridovich I. Mechanism of the antibiotic action pyocyanine[J]. Journal of bacteriology, 1980, 141(1): 156-163.
[3] Jayaseelan S, Ramaswamy D, Dharmaraj S. Pyocyanin: production, applications, challenges and new insights[J]. World Journal of Microbiology and Biotechnology, 2014, 30(4): 1159-1168.
[4] Rada B, Leto T L. Pyocyanin effects on respiratory epithelium: relevance in Pseudomonas aeruginosa airway infections[J]. Trends in microbiology, 2013, 21(2): 73-81.
[5] Caldwell C C, Chen Y, Goetzmann H S, et al. Pseudomonas aeruginosa exotoxin pyocyanin causes cystic fibrosis airway pathogenesis[J]. The American journal of pathology, 2009, 175(6): 2473-2488.
[6] Muller M. Premature cellular senescence induced by pyocyanin, a redox-active Pseudomonas aeruginosa toxin[J]. Free Radical Biology and Medicine, 2006, 41(11): 1670-1677.
[7] Eltarahony M M, Younis S S, Abdel Salam S A, et al. An insight into pyocyanin: production, characterization, and evaluation of its in vitro antibacterial, antifungal, antibiofilm and in vivo anti-schistosomal potency[J]. BMC microbiology, 2025, 25(1): 532.
| Cell experiment [1]: | |
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Cell lines |
NCI-H292 cells |
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Preparation Method |
NCI-H292 cells were treated with 25μg/mL Pyocyanin for 24h, then total cellular RNA was extracted and subjected to qRT-PCR analysis using specific primers for MUC5AC and GAPDH (as internal control) to assess MUC5AC mRNA expression levels. |
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Reaction Conditions |
25μg/mL; 24h |
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Applications |
Treatment with Pyocyanin induced an approximately 4-fold increase in the transcriptional level of the mucin synthesis gene MUC5AC. |
| Animal experiment [2]: | |
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Animal models |
Swiss albino mice infected with S. mansoni |
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Preparation Method |
Swiss albino mice infected with S. mansoni cercariae were orally given Pyocyanin (25mg/kg/day) for 5 days from day 21 post-infection. On day 49, adult worms were recovered by perfusion and counted to calculate worm reduction. |
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Dosage form |
25mg/kg/day; 5 days; p.o. |
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Applications |
Pyocyanin was used to treat mice for 5 days starting on day 21 of infection with S. mansoni. At day 49, the total worm burden (23.83 ± 2.79) was significantly lower than that of the control group (36.33 ± 3.98), with a reduction rate of 34.41%. |
| References: [1] Caldwell C C, Chen Y, Goetzmann H S, et al. Pseudomonas aeruginosa exotoxin pyocyanin causes cystic fibrosis airway pathogenesis[J]. The American journal of pathology, 2009, 175(6): 2473-2488. [2] Eltarahony M M, Younis S S, Abdel Salam S A, et al. An insight into pyocyanin: production, characterization, and evaluation of its in vitro antibacterial, antifungal, antibiofilm and in vivo anti-schistosomal potency[J]. BMC microbiology, 2025, 25(1): 532. | |
| Cas No. | 85-66-5 | SDF | |
| Synonyms | Sanasin,Sanazin,Pyocyanine | ||
| Chemical Name | 5-methyl-1(5H)-phenazinone | ||
| Canonical SMILES | Cn1c2ccccc2nc2c(=O)cccc12 | ||
| Formula | C13H10N2O | M.Wt | 210.2 |
| Solubility | 5mg/ml in ethanol, or in DMSO; 2.5mg/ml in DMF | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.7574 mL | 23.7869 mL | 47.5737 mL |
| 5 mM | 951.5 μL | 4.7574 mL | 9.5147 mL |
| 10 mM | 475.7 μL | 2.3787 mL | 4.7574 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 18 reference(s) in Google Scholar.)