|Pyocyanin Catalog No.GC13137|
Sample solution is provided at 25 µL, 10mM.
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|Solubility||5mg/ml in ethanol, or in DMSO; 2.5mg/ml in DMF||Storage||Store at -20°C|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|
|Shipping Condition||Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
Pyocyanin (PCN) is a blue redox-active secondary metabolite produced by Pseudomonas aeruginosa. PCN is readily recovered in large quantities in sputum from patients patients infected with P. aeruginosa . PCN interferes with multiple cellular functions .
In vitro: Pyocyanin induced a time- and concentration-dependent acceleration of neutrophil apoptosis. Treatment with 50 μM pyocyanin resulted in a 10-fold induction of apoptosis at 5h(p < 0.001). The concentration 50 (μM) has been documented in sputum from patients colonized with P. aeruginosa. Pyocyanin showed no effects on the apoptosis of monocyte-derived macrophages or airway epithelial cells at time points up to 24 h . Pyocyanin resulted in gradual onset of slowing and ultimate widespread ciliostasis with epithelial disruption .
In vivo: In a murine model of acute pneumonia, pyocyanin production by P. aeruginosa suppressed the acute inflammatory response by pathogen-driven acceleration of neutrophil apoptosis and by reducing local inflammation. Intratracheal instillation of wild-type and pyocyanin-deficient strains of P. aeruginosa caused a rapid increase in bronchoalveolar lavage neutrophil counts up to 18 h after infection.
In wild-type infection, the number of neutrophil declined steadily. neutrophil numbers increased up to 48 h in mice infected with pyocyanin-deficient P. aeruginosa. Pyocyanin production was associated with reduced bacterial clearance from the lungs. The concentration of potent neutrophil chemokines (MIP-2, KC) and cytokines (IL-6, IL-1β) were significantly lower in wild-type when compared with that of phenazine-deficient strain-infected mice at 18 h . Pyocyanin (600 ng) resulted in 60% reduction in tracheal mucus velocity at 3 h, and no recovery occurred .
 Lau G W, Hassett D J, Ran H, et al. The role of pyocyanin in Pseudomonas aeruginosa infection[J]. Trends in molecular medicine, 2004, 10(12): 599-606.
 Usher L R, Lawson R A, Geary I, et al. Induction of neutrophil apoptosis by the Pseudomonas aeruginosa exotoxin pyocyanin: a potential mechanism of persistent infection[J]. The Journal of Immunology, 2002, 168(4): 1861-1868.
 Wilson R, Pitt T, Taylor G, et al. Pyocyanin and 1-hydroxyphenazine produced by Pseudomonas aeruginosa inhibit the beating of human respiratory cilia in vitro[J]. Journal of Clinical Investigation, 1987, 79(1): 221.
 Allen L, Dockrell D H, Pattery T, et al. Pyocyanin production by Pseudomonas aeruginosa induces neutrophil apoptosis and impairs neutrophil-mediated host defenses in vivo[J]. The Journal of Immunology, 2005, 174(6): 3643-3649.
 Munro N C, Barker A, Rutman A, et al. Effect of pyocyanin and 1-hydroxyphenazine on in vivo tracheal mucus velocity[J]. Journal of applied physiology, 1989, 67(1): 316-323.