|Pertussis Toxin Catalog No.GC17532|
Sample solution is provided at 25 µL, 10mM.
|Solubility||Each vial, when reconstituted to 500 μl with water, contains 50 μg of pertussis toxin in 0.03 M sodium phosphate, 0.05 M sodium chloride, pH 7.0||Storage||Store at 2-8°C|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|
|Shipping Condition||Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
Pertussis toxin (islet-activating protein) is a toxin, first isolated from B. pertussis, that is used to study G protein-coupled receptor signaling in cells and experimental autoimmune encephalomyelitis (EAE) in animals. Pertussis toxin catalyzes the transfer of the ADP-ribose moiety of NAD to the α subunits of heterotrimeric Gi/o proteins, resulting in the receptors being uncoupled from Gi/o proteins., Pertussis toxin is also used as an adjuvant, given with specific antigens, to immunize animals and induce EAE, an animal model of multiple sclerosis., Pertussis toxin was first described as an islet-activating protein because it caused a sustained potentiation of the secretory response of pancreatic islet cells to various stimuli that stimulate Gi-linked α-adrenergic receptors.,
. Kaslow, H.R., and Burns, D.L. Pertussis toxin and target eukaryotic cells: Binding, entry, and activation. FASEB J. 6(9), 2684-2690 (1992).
. Ui, M. Islet-activating protein, pertussis toxin: A probe for functions of the inhibitory guanine nucleotide regulatory component of adenylate cyclase. Trends Pharmacol. Sci. 5, 277-279 (1984).
. Hofstetter, H.H., Shive, C.L., and Forsthuber, T.G. Pertussis toxin modulates the immune response to neuroantigens injected in incomplete Freund’s adjuvant: Induction of Th1 cells and experimental autoimmune encephalomyelitis in the presence of high frequencies of Th2 cells. Journal of Immunology 169(1), 117-125 (2002).
. Ronchi, F., Basso, C., Preite, S., et al. Experimental priming of encephalitogenic Th1/Th17 cells requires pertussis toxin-driven IL-1β production by myeloid cells. Nat.Commun. 7:11541, (2016).
. Heyworth, C.M., Grey, A.M., Wilson, S.R., et al. The action of islet activating protein (pertussis toxin) on insulin’s ability to inhibit adenylate cyclase and activate cyclic AMP phosphodiesterases in hepatocytes. Biochemistry Journal 235(1), 145-149 (1986).
. Katada, T., and Michio, U. Slow interaction of islet-activating protein with pancreatic islets during primary culture to cause reversal of α-adrenergic inhibition of insulin secretion. The Journal of Biological Chemisty 255(20), 9580-9588 (1980).