SA72 |
Catalog No.GC31239
One-Click Copy Product Info
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SA72 is a highly selective fatty acid amide hydrolase (FAAH) inhibitor.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 934809-60-6
Sample solution is provided at 25 µL, 10mM.
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SA72 is a highly selective fatty acid amide hydrolase (FAAH) inhibitor.
Fatty acid amide hydrolase (FAAH) is the primary regulator of several bioactive lipid amides including anandamide. Inhibitors of FAAH are potentially useful for the treatment of pain, anxiety, depression, and other nervous system disorders. However, FAAH inhibitors must display selectivity for this enzyme relative to the numerous other serine hydrolases present in the human proteome in order to be therapeutically acceptable. SA-72 is also a carbamate inhibitor. However, SA-72 shows exceptional selectivity for FAAH. Tested carboxylesterases are not off-targets for SA-72. SA-72 does not have major effects on the level of carboxylesterase activities in liver microsomes[1].
[1]. Zhang D, et al. Fatty acid amide hydrolase inhibitors display broad selectivity and inhibit multiple carboxylesterases as off-targets. Neuropharmacology. 2007 Mar;52(4):1095-105.
Kinase experiment: | Rat liver microsomes and cytosol are as sources of carboxylesterase to screen activity. Assays are conducted in 96-well microtiter plates at room temperature. Briefly, 0.5 mg liver microsomes or 2 mg cytosol are incubated with 10 mM SA72 for 30 min and then substrate p-nitrophenyl acetate is added to a final concentration of 1 mM. The plate is read 10 min after substrate addition at 405 nm for the appearance of the p-nitrophenol. The carboxylesterase activities in liver extracts without inhibitor added are set at 100% and the remaining carboxylesterase activities after incubation with inhibitors are calculated relative to the control[1]. |
References: [1]. Zhang D, et al. Fatty acid amide hydrolase inhibitors display broad selectivity and inhibit multiple carboxylesterases as off-targets. Neuropharmacology. 2007 Mar;52(4):1095-105. | |
| Cas No. | 934809-60-6 | SDF | |
| Canonical SMILES | O=C(OCC(N)=O)NCCCC1OCC(C2=C3C=CC(OC)=CC3=CC=C2)CO1 | ||
| Formula | C21H26N2O6 | M.Wt | 402.44 |
| Solubility | Soluble in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.4848 mL | 12.4242 mL | 24.8484 mL |
| 5 mM | 497 μL | 2.4848 mL | 4.9697 mL |
| 10 mM | 248.5 μL | 1.2424 mL | 2.4848 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 39 reference(s) in Google Scholar.)