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C6 NBD Ceramide (d18:1/6:0) Catalog No.GC43096

A biologically active, fluorescent analog of ceramide

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Sample solution is provided at 25 µL, 10mM.

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Chemical Properties

Cas No. 94885-02-6 SDF Download SDF
Synonyms C6 NBD Ceramide, NBD Cer(18:1/2:0), NBD Ceramide (d18:1/6:0) , N-C6-NBD-D-erythro-Sphingosine
Chemical Name N-[7-(4-nitrobenzo-2-oxa-1,3-diazole)]-6-aminocaproyl-D-erythro-sphingosine
Canonical SMILES CCCCCCCCCCCCC/C=C/[C@@H](O)[C@@H](NC(CCCCCN(C1=CC=C([N+]([O-])=O)C2=NON=C12)[H])=O)CO
Formula C30H49N5O6 M.Wt 575.7
Solubility 5mg/mL in ethanol; 10mg/mL in DMSO; 25mg/mL in DMF Storage Store at -20°C
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
Shipping Condition Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
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C6 NBD ceramide is a biologically active fluorescent analog of short chain, membrane-permeable ceramides.1 It is as effective as C6 ceramide in the inhibition of viral glycoprotein transport through the Golgi.[1] C6 NBD ceramide has been used as a fluorescent substrate for the activity of UDP-glucose:ceramide glucosyltransferase and to demonstrate the translocation of glucocerebroside and sphingomyelin from the Golgi to the plasma membrane.[2],[3]

[1]. Rosenwald, A.G., and Pagano, R.E. Inhibition of glycoprotein traffic through the secretory pathway by ceramide. J. Biol. Chem. 268(7), 4577-4579 (1993).
[2]. Paul, P., Kamisaka, Y., Marks, D.L., et al. Purification and characterization of UDP-glucose: Ceramide glucosyltransferase from rat liver Golgi membranes. J. Biol. Chem. 271(4), 2287-2293 (1996).
[3]. Lipsky, N.G., and Pagano, R.E. Intracellular translocation of fluorescent sphingolipids in cultured fibroblasts: Endogenously synthesized sphingomyelin and glucocerebroside analogues pass through the golgi apparatus en route to the plasma membrane. J. Cell. Biol. 100(1), 27-34 (1985).