7-Azido-4-methylcoumarin (Synonyms: AzMC) |
Catalog No.GC45707
One-Click Copy Product Info
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7-Azido-4-methylcoumarin is a highly sensitive and selective fluorescent hydrogen sulfide probe (excitation wavelength: 365nm; emission wavelength: 450nm).
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 95633-27-5
Sample solution is provided at 25 µL, 10mM.
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7-Azido-4-methylcoumarin is a highly sensitive and selective fluorescent hydrogen sulfide probe (excitation wavelength: 365nm; emission wavelength: 450nm). In the presence of H₂S, the aromatic azido moiety of 7-Azido-4-methylcoumarin can be selectively reduced. 7-Azido-4-methylcoumarin can participate in copper-catalyzed azide-alkyne cycloaddition (CuAAC) and strain-promoted azide-alkyne cycloaddition (SPAAC). 7-Azido-4-methylcoumarin can be used in research related to hydrogen sulfide detection and PLP-dependent enzyme activity monitoring[1-4].
References:
[1] Thorson MK, Majtan T, Kraus JP, et al. Identification of cystathionine β-synthase inhibitors using a hydrogen sulfide selective probe. Angew Chem Int Ed Engl. 2013 Apr 22;52(17):4641-4.
[2] Módis K, Coletta C, Asimakopoulou A, et al. Effect of S-adenosyl-L-methionine (SAM), an allosteric activator of cystathionine-β-synthase (CBS) on colorectal cancer cell proliferation and bioenergetics in vitro. Nitric Oxide. 2014 Sep 15;41:146-56.
[3] Wang H, Jia M, Chang Y, et al. Hydrogen sulfide donor NaHS inhibits formaldehyde-induced epithelial-mesenchymal transition in human lung epithelial cells via activating TGF-β1/Smad2/3 and MAPKs signaling pathways. Curr Res Toxicol. 2024 Oct 24;7:100199.
[4] Chang GQ, Bai SZ, Sun FQ, et al. SKF38393 prevents high glucose (HG)-induced endothelial dysfunction by inhibiting the effects of HG on cystathionine γ-lyase/hydrogen sulfide activity and via a RhoA/ROCK1 pathway. Front Biosci (Landmark Ed). 2022 Feb 11;27(2):49.
7-Azido-4-methylcoumarin method for detecting hydrogen sulfide content in cells[1]
This protocol is adapted from research data and provided for reference only. Adjustments may be necessary based on specific experimental requirements.
1. Reagent Preparation: Dissolve 7-Azido-4-methylcoumarin in PBS to prepare a 50μM working solution.
2. Cell Culture: Culture cells in appropriate medium containing 10% fetal bovine serum at a seeding density of 5×10⁴ cells/cm², and incubate at 37°C under 5% CO₂ conditions.
3. Hydrogen Sulfide Detection: Incubate cells with the 50μM 7-Azido-4-methylcoumarin working solution at 37°C in the dark for 30 minutes, then wash the cells three times with PBS. Observe the intracellular fluorescence intensity using a fluorescence microscope (ex=365nm; em=450nm).
Precautions:
(1) For your safety and health, please wear a lab coat and disposable gloves during operation.
References:
[1] Liu N, Wu J, Zhang L, et al. Hydrogen Sulphide modulating mitochondrial morphology to promote mitophagy in endothelial cells under high-glucose and high-palmitate. J Cell Mol Med. 2017 Dec;21(12):3190-3203.
| Cas No. | 95633-27-5 | SDF | |
| Synonyms | AzMC | ||
| Canonical SMILES | O=C(C=C1C)OC2=C1C=CC(N=[N+]=[N-])=C2 | ||
| Formula | C10H7N3O2 | M.Wt | 201.2 |
| Solubility | DMSO : 50 mg/mL (248.53 mM; Need ultrasonic) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.9702 mL | 24.8509 mL | 49.7018 mL |
| 5 mM | 994 μL | 4.9702 mL | 9.9404 mL |
| 10 mM | 497 μL | 2.4851 mL | 4.9702 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 20 reference(s) in Google Scholar.)