8pyDTZ |
| Catalog No.GC39334 |
8pyDTZ is a pyridyl diphenylterazine (DTZ) analog designed for bioluminescent imaging, specifically optimized for use with the LumiLuc luciferase system.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 2351898-91-2
Sample solution is provided at 25 µL, 10mM.
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8pyDTZ is a pyridyl diphenylterazine (DTZ) analog designed for bioluminescent imaging, specifically optimized for use with the LumiLuc luciferase system. In the presence of pyridyl substrates 8pyDTZ, LumiLuc generates highly bright yellow bioluminescence. 8pyDTZ offers enhanced water solubility and red-shifted emission, making it suitable for in vivo imaging applications[1]. 8pyDTZ has about 13 times better aqueous solubility and bioavailability than DTZ. The emission from 8-pyDTZ/LumiLuc was about 5 times brighter than 8-pyDTZ/teLuc[2].
In vitro, the 8pyDTZ (30µM) can been used to quantify biomarkers such as human serum albumin (HSA) in serum samples and α-amylase in saliva samples by incubating the analyte of interest for 30min at 25℃[3].
In vivo, 8pyDTZ (0.2μmol/mouse) in 100μL normal saline is administrated into luciferase expressing HeLa cells xenograft mouse model via intravenous delivery to track tumor growth with high sensitivity and good tissue penetration ability[1].
References:
[1] Yeh H W, Xiong Y, Wu T C, et al. ATP-Independent Bioluminescent Reporter Variants To Improve in Vivo Imaging. ACS Chem Biol. 2019 May 17;14(5):959-965.
[2] Syed A J, Anderson J C. Applications of bioluminescence in biotechnology and beyond. Chem Soc Rev. 2021 May 7;50(9):5668-5705.
[3] Guo Z, Parakra R D, Xiong Y, et al. Engineering and exploiting synthetic allostery of NanoLuc luciferase. Nat Commun. 2022 Feb 10;13(1):789.
The use of 8pyDTZ in HEK 293T cells to evaluate its bioluminescence performance：
1. Culture HEK 293T cells in DMEM with 10% FBS at 37°C with 5% CO2.
2. Perfom transfection when cell confluency reaches 70-80%.
3. Transiently transfect cells with the LumiLuc luciferase expression plasmid using liposome-based transfection
4. The expression of LumiLuc luciferase in the cells was detected 24-48 hours after transfection.
5. Dissolve 8pyDTZ in ethanol to a concentration of 10mM.
6. Use an assay buffer composed of 1mM CDTA, 0.5% Tergitol NP-40, 0.05% Antifoam 204, 150mM KCl, 100mM MES pH 6.0, 1mM DTT, and 35mM thiourea.
7. Dilute the 8pyDTZ stock solution (10mM) in the assay buffer to final concentration of 20µM.
8. Seed transfected cells into 96-well plate at a density of 5,000 cells/well.
9. Allow cells to adhere and recover for 2-4 hours in the incubator.
10. Add 50µL of the 8pyDTZ (20µM) containing solution to each well containing the transfected cells.
11. Incubate the cells with 8pyDTZ at 37°C for 10 minutes.
12. Perform bioluminescence imaging immediately after incubation using a luminescence plate reader with exposure time of 2s.
13. Use a 600-700nm bandpass filter to capture far-red emission.
This protocol only provides a guideline, and should be modified according to your specific needs.
References:
[1] Yeh H W, Xiong Y, Wu T C, et al. ATP-Independent Bioluminescent Reporter Variants To Improve in Vivo Imaging. ACS Chem Biol. 2019 May 17;14(5):959-965.
| Cas No. | 2351898-91-2 | SDF | |
| Canonical SMILES | O=C1C(CC2=CC=CC=C2)=NC3=C(C4=CC=NC=C4)NC(C5=CC=CC=C5)=CN31 | ||
| Formula | C24H18N4O | M.Wt | 378.43 |
| Solubility | DMSO: 12.5 mg/mL (33.03 mM; DMSO can inactivate 8pyDTZ's activity); EtOH+HCl: 2 mg/mL (5.28 mM; ultrasonic and adjust pH to 2 with 1M HCl; DMSO can inactivate 8pyDTZ's activity); Water: < 0.1 mg/mL (insoluble; DMSO can inactivate 8pyDTZ's activity) | Storage | Store at -20°C,unstable in solution, ready to use. |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.6425 mL | 13.2125 mL | 26.425 mL |
| 5 mM | 528.5 μL | 2.6425 mL | 5.285 mL |
| 10 mM | 264.2 μL | 1.3212 mL | 2.6425 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 8 reference(s) in Google Scholar.)
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