AA 147 |
Catalog No.GC50566
One-Click Copy Product Info
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AA 147 is a small molecule endoplasmic reticulum (ER) proteostasis regulator.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 393121-74-9
Sample solution is provided at 25 µL, 10mM.
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AA 147 is a small molecule endoplasmic reticulum (ER) proteostasis regulator. AA 147 can selectively activate the ATF6 arm of the unfolded protein response (UPR) and activate the NRF2 oxidative stress response. AA 147 can be used in research related to neurodegenerative diseases, cardiovascular diseases, and multiple sclerosis[1-4].
In vitro, AA 147 (10μM) pretreated THP1 cells and RAW264.7 mouse macrophages for 16 hours, followed by stimulation with LPS (1μg/mL) and nigericin (10μM) or ATP (5mM) for 3-24 hours. AA 147 significantly inhibited the expression of the pro-inflammatory cytokine IL-1β and reduced the inflammatory response[5]. AA 147 (10μM) treated HT22 cells for 40 hours. AA 147 protected HT22 cells against glutamate-induced oxytosis and erastin-induced ferroptosis[6].
In vivo, AA 147 (2mg/kg) was administered intraperitoneally 1 day before surgery and intravenously 15 minutes after the return of spontaneous circulation, for the treatment of C57BL/6 mice that underwent 9 minutes of cardiac arrest surgery and cardiopulmonary resuscitation. AA 147 significantly improved neurological outcomes and reduced neuronal death[7]. AA 147 (2mg/kg) was administered intraperitoneally 1 hour after stroke, followed by daily injections for 5 days, for the treatment of young (3-4 months old) and aged (18-20 months old) C57BL/6 mice that underwent permanent middle cerebral artery occlusion. AA 147 significantly improved the mice's performance in neurological scores, gait analysis, tape removal test, and Y-maze test, and promoted long-term functional recovery in the mice[8].
References:
[1] Rosarda JD, Baron KR, Nutsch K, et al. Metabolically Activated Proteostasis Regulators Protect against Glutamate Toxicity by Activating NRF2. ACS Chem Biol. 2021 Dec 17;16(12):2852-2863.
[2] Kubra KT, Akhter MS, Saini Y, et al. Activating transcription factor 6 protects against endothelial barrier dysfunction. Cell Signal. 2022 Nov;99:110432.
[3] D'Amico D, Biondi O, Januel C, et al. Activating ATF6 in spinal muscular atrophy promotes SMN expression and motor neuron survival through the IRE1α-XBP1 pathway. Neuropathol Appl Neurobiol. 2022 Aug;48(5):e12816.
[4] Li B, Yang H, Wu H, et al. Hydroquinone-induced endoplasmic reticulum stress affects TK6 cell autophagy and apoptosis via ATF6-mTOR. Environ Toxicol. 2023 Aug;38(8):1874-1890.
[5] Rosarda JD, Stanton CR, Chen EB, et al. Pharmacologic Targeting of PDIA1 Inhibits NLRP3 Inflammasome Assembly and Activation. Isr J Chem. 2024 Dec;64(12):e202300125.
[6] Kline GM, Madrazo N, Cole CM, et al. Metabolically activated proteostasis regulators that protect against erastin-induced ferroptosis. RSC Chem Biol. 2024 Jul 17;5(9):866-876.
[7] Yuan Z, Lu L, Lian Y, et al. AA147 ameliorates post-cardiac arrest cerebral ischemia/reperfusion injury through the co-regulation of the ATF6 and Nrf2 signaling pathways. Front Pharmacol. 2022 Nov 23;13:1028002.
[8] Yu X, Dang L, Dhar A, et al. Activation of ATF6 Signaling Confers Long-Term Beneficial Effects in Young and Aged Mice After Permanent Stroke. Transl Stroke Res. 2025 Oct;16(5):1799-1810.
| Cell experiment [1]: | |
Cell lines | HT22 cells (mouse hippocampal neuronal cell line) |
Preparation Method | HT22 cells were pretreated with AA 147 (10μM) for 16 hours prior to challenge with glutamate or erastin. |
Reaction Conditions | 10μM; 16h |
Applications | AA 147 protected HT22 cells against glutamate-induced oxytosis and erastin-induced ferroptosis. AA 147 activated the NRF2 oxidative stress response in neuronal cells. |
| Animal experiment [2]: | |
Animal models | C57BL/6 mice (young 3-4 months old and aged 18-20 months old) |
Preparation Method | AA 147 (2mg/kg) was administered intraperitoneally at 1 hour after permanent middle cerebral artery occlusion (pMCAO), followed by daily injections for 5 days. |
Dosage form | 2mg/kg; i.p.; daily injections for 5 days |
Applications | AA 147 treatment significantly improved long-term functional recovery, including neurologic scores, gait analysis, tape removal test, and Y-maze test performance in both young and aged mice after permanent stroke. |
References: | |
| Cas No. | 393121-74-9 | SDF | |
| Canonical SMILES | CC1=CC(NC(CCC2=CC=CC=C2)=O)=C(C=C1)O | ||
| Formula | C16H17NO2 | M.Wt | 255.31 |
| Solubility | DMSO : 50 mg/mL (195.84 mM; Need ultrasonic) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.9168 mL | 19.584 mL | 39.1681 mL |
| 5 mM | 783.4 μL | 3.9168 mL | 7.8336 mL |
| 10 mM | 391.7 μL | 1.9584 mL | 3.9168 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.50% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 2 reference(s) in Google Scholar.)