Abz-Val-Ala-Asp-Nva-Arg-Asp-Arg-Gln-EDDnp (trifluoroacetate salt) |
Catalog No.GC42684
One-Click Copy Product Info
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Abz-Val-Ala-Asp-Nva-Arg-Asp-Arg-Gln-EDDnp (trifluoroacetate salt) is a modified peptide containing fluorescence resonance energy transfer (FRET) groups.
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
Abz-Val-Ala-Asp-Nva-Arg-Asp-Arg-Gln-EDDnp (trifluoroacetate salt) is a modified peptide containing fluorescence resonance energy transfer (FRET) groups. Fluorescence signals can be monitored using an excitation wavelength of 320nm and an emission wavelength of 420nm, making Abz-Val-Ala-Asp-Nva-Arg-Asp-Arg-Gln-EDDnp commonly used as a fluorescent substrate for detecting protease activity[1].
References:
[1] Yu J, Fu Y, Gao J, et al. Cathepsin C from extracellular histone-induced M1 alveolar macrophages promotes NETosis during lung ischemia-reperfusion injury. Redox Biol. 2024 Aug;74:103231.
Assay of Proteasome Activity in Cell
This protocol is adapted from research data and provided for reference only. Adjustments may be necessary based on specific experimental requirements.
(1) Reagent Preparation: Dissolve the Abz-Val-Ala-Asp-Nva-Arg-Asp-Arg-Gln-EDDnp lyophilized powder in dimethylformamide (DMF) to prepare a high-concentration stock solution (e.g., 5mM), then aliquot and store at -20°C.
(2) Enzyme Sample Preparation: Use purified PR3 enzyme (typically at concentrations ranging from 0.1nM to 10nM) or cell suspensions containing PR3 (e.g., activated neutrophils).
(3) PR3 enzyme Activity Detection: The reaction mixture contains 20μM Abz-Val-Ala-Asp-Nva-Arg-Asp-Arg-Gln-EDDnp, 50mM HEPES, 150mM NaCl, and 0.05% Triton X-100, with a total volume of 200μL. Incubate at 37°C for 30 minutes. Monitor fluorescence intensity using a microplate spectrofluorometer. The excitation wavelength for Abz is 320nm, and the emission wavelength is 420nm.
Precautions:
(1) Free fluorescent substrates are subject to quenching. Please try to avoid light exposure to slow down fluorescence quenching.
(2) DMF may affect enzyme activity. The final concentration of DMF in the stock solution should be kept as low as possible (typically <1%).
(3) For your safety and health, please wear a lab coat and disposable gloves when operating.
References:
[1] Korkmaz B, Attucci S, Epinette C, et al. Measurement of neutrophil elastase, proteinase 3, and cathepsin G activities using intramolecularly quenched fluorogenic substrates. Methods Mol Biol. 2012;844:125-38.
| Cas No. | SDF | ||
| Canonical SMILES | O=C(NCCNC1=CC=C([N+]([O-])=O)C=C1[N+]([O-])=O)[C@@H](NC([C@@H](NC([C@@H](NC([C@@H](NC([C@H](CCC)NC([C@@H](NC([C@@H](NC([C@@H](NC(C2=CC=CC=C2N)=O)C(C)C)=O)C)=O)CC(O)=O)=O)=O)CCCNC(N)=N)=O)CC(O)=O)=O)CCCNC(N)=N)=O)CCC(N)=O.FC(F)(C(O)=O)F | ||
| Formula | C53H80N20O18•XCF3COOH | M.Wt | 1285.3 |
| Solubility | DMF:Water: Soluble,Water: Soluble | Storage | Store at -20°C; protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 778 μL | 3.8901 mL | 7.7803 mL |
| 5 mM | 155.6 μL | 778 μL | 1.5561 mL |
| 10 mM | 77.8 μL | 389 μL | 778 μL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.50% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 33 reference(s) in Google Scholar.)