α-Estradiol (Synonyms: Alfatradiol, α-Estradiol, 17-epi Estradiol, NSC 20293, 17α-Oestradiol) |
Catalog No.GC15975
One-Click Copy Product Info
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α-Estradiol, a natural form of the hormone lacking estrogenic effects, inhibits the activity of 5-alpha reductase enzymes.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 57-91-0
Sample solution is provided at 25 µL, 10mM.
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α-Estradiol, a natural form of the hormone lacking estrogenic effects, inhibits the activity of 5-alpha reductase enzymes [1]. α-Estradiol can cross the blood-brain barrier and bind to the brain-specific estrogen receptor ER-X, thereby influencing the regulation of energy metabolism in the central nervous system [2]. α-Estradiol has been widely used to extend the lifespan of male mice and reduce the neuroinflammatory response in the hypothalamus of mice[3].
In vitro, α-Estradiol (10nM) treatment for 24 hours significantly inhibited H2O2-induced cell death in SN4741 cells[4]. Treatment with 100nM α-Estradiol for 24 hours significantly reduced the death of PC12 cells induced by 1-methyl-4-phenylpyridinium (MPP) and restored the expression of dopamine transporter proteins[5].
In vivo, α-Estradiol treatment via oral administration at a dose of 4.8mg/kg daily for 12 months significantly prolonged the lifespan of male mice[6]. Oral administration of α-Estradiol at a dose of 14.4mg/kg/day for 8 months significantly altered hepatic metabolism and increased the levels of multiple amino acids in male mice [1]. Oral administration of α-Estradiol at a dose of 14.4mg/kg daily for 6 months significantly improved skeletal muscle and myofiber phenotype as well as physical function in male mice[7].
References:
[1] Garratt M, Lagerborg K A, Tsai Y M, et al. Male lifespan extension with 17‐α estradiol is linked to a sex‐specific metabolomic response modulated by gonadal hormones in mice[J]. Aging Cell, 2018, 17(4): e12786.
[2] Garratt M, Bower B, Garcia G G, et al. Sex differences in lifespan extension with acarbose and 17‐α estradiol: gonadal hormones underlie male‐specific improvements in glucose tolerance and mTORC 2 signaling[J]. Aging cell, 2017, 16(6): 1256-1266.
[3] Debarba L K, Jayarathne H S M, Miller R A, et al. 17-α-Estradiol has sex-specific effects on neuroinflammation that are partly reversed by gonadectomy[J]. The Journals of Gerontology: Series A, 2022, 77(1): 66-74.
[4] Biewenga E, Cabell L, Audesirk T. Estradiol and raloxifene protect cultured SN4741 neurons against oxidative stress[J]. Neuroscience letters, 2005, 373(3): 179-183.
[5] Gélinas S, Martinoli M G. Neuroprotective effect of estradiol and phytoestrogens on MPP+‐induced cytotoxicity in neuronal PC12 cells[J]. Journal of Neuroscience Research, 2002, 70(1): 90-96.
[6] Harrison D E, Strong R, Allison D B, et al. Acarbose, 17‐α‐estradiol, and nordihydroguaiaretic acid extend mouse lifespan preferentially in males[J]. Aging cell, 2014, 13(2): 273-282.
[7] Garratt M, Leander D, Pifer K, et al. 17‐α estradiol ameliorates age‐associated sarcopenia and improves late‐life physical function in male mice but not in females or castrated males[J]. Aging Cell, 2019, 18(2): e12920.
| Cell experiment [1]: | |
Cell lines | SN4741 cells |
Preparation Method | SN4741 cells were cultured in MEM medium buffered with 10mM NaHCO3 and 25mM HEPES, supplemented with 2mM l-glutamine, 2% glucose, 1mM sodium pyruvate, 10% fetal bovine serum, 8% dimethyl sulfoxide (DMSO), and 15mM KCl. After 24h, cells had multiplied to near confluence, the medium was replaced with culture medium supplemented with 0.5% fetal bovine serum. Cells were incubated at 40°C in α-Estradiol (10nM), raloxifene (10nM), or ICI 182,780 (100nM) dissolved in DMSO. The control cell received an equal amount of DMSO. After 24h of incubation withα-Estradiol, H2O2 was added (50μM) and allowed to remain on the cultures for 24h, the cell viability was analyzed. |
Reaction Conditions | 10nM; 24h |
Applications | α-Estradiol treatment significantly inhibited H2O2-induced cell death in SN4741 cells. |
| Animal experiment [2]: | |
Animal models | UM-HET3 mice |
Preparation Method | UM-HET3 mice (16 months old) were maintained under a 12h light and 12h dark cycle at a temperature of 25°C and humidity of 50%±10%, free with water and diet. The mice were fed with a diet containing 14.4mg/kg of α-Estradiol every day for 6 months, and the motor abilities were analyzed. |
Dosage form | 14.4mg/kg/day; 6 months; p.o. |
Applications | α-Estradiol treatment improved rotarod balance capacity and endurance capacity in mice. |
References: | |
| Cas No. | 57-91-0 | SDF | |
| Synonyms | Alfatradiol, α-Estradiol, 17-epi Estradiol, NSC 20293, 17α-Oestradiol | ||
| Chemical Name | (8R,9S,13S,14S,17R)-13-methyl-7,8,9,11,12,13,14,15,16,17-decahydro-6H-cyclopenta[a]phenanthrene-3,17-diol | ||
| Canonical SMILES | O[C@H]1[C@]2(C)[C@@H](CC1)[C@H](CCC3=C4C=CC(O)=C3)[C@@H]4CC2 | ||
| Formula | C18H24O2 | M.Wt | 272.38 |
| Solubility | ≥ 13.15mg/mL in Ethanol | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.6713 mL | 18.3567 mL | 36.7134 mL |
| 5 mM | 734.3 μL | 3.6713 mL | 7.3427 mL |
| 10 mM | 367.1 μL | 1.8357 mL | 3.6713 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 24 reference(s) in Google Scholar.)