Bikinin (Synonyms: Abrasin) |
Catalog No.GC10752
One-Click Copy Product Info
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Bikinin is a non-steroidal, ATP-competitive inhibitor of plant GSK-3 (glycogen synthase kinase 3)/Shaggy-like kinases.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 188011-69-0
Sample solution is provided at 25 µL, 10mM.
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Bikinin is a non-steroidal, ATP-competitive inhibitor of plant GSK-3 (glycogen synthase kinase 3)/Shaggy-like kinases. Bikinin can be used for research on plant growth and development, hormone signal transduction, and abiotic stress response mechanism[1-4].
In vitro, barley (Golden Promise and Haruna Nijo cultivars) was treated with Bikinin (10–100μM) for 0–5 days under salt stress conditions, followed by continuous stimulation with 150mM sodium chloride (NaCl) until the BBCH20 growth stage. Bikinin significantly reduced the abundance of phosphorylated HvBZR1 protein (inhibiting its phosphorylation level), while upregulating the expression of genes related to the BR signaling pathway and alleviating salt stress-induced damage to plant cells[5]. Bikinin (25–50μM) was administered to pretreat wild-type Marchantia polymorpha (Tak-1 cultivar) plants for 0–5 days, followed by normal cultivation for 5–10 days. Bikinin significantly promoted cell proliferation activity in the apical meristematic region, expanded the boundaries of this region, and simultaneously inhibited subsequent cell differentiation, leading to the loss of the typical dorsoventral structure and the accumulation of undifferentiated cell masses[6].
References:
[1] Deng XG, Zhu T, Peng XJ, et al. Role of brassinosteroid signaling in modulating Tobacco mosaic virus resistance in Nicotiana benthamiana. Sci Rep. 2016 Feb 3;6:20579.
[2] Rozhon W, Wang W, Berthiller F, et al. Bikinin-like inhibitors targeting GSK3/Shaggy-like kinases: characterisation of novel compounds and elucidation of their catabolism in planta. BMC Plant Biol. 2014 Jun 19;14:172.
[3] De Rybel B, Audenaert D, Vert G, et al. Chemical inhibition of a subset of Arabidopsis thaliana GSK3-like kinases activates brassinosteroid signaling. Chem Biol. 2009 Jun 26;16(6):594-604.
[4] Lu Q, Houbaert A, Ma Q, et al. Adenosine monophosphate deaminase modulates BIN2 activity through hydrogen peroxide-induced oligomerization. Plant Cell. 2022 Sep 27;34(10):3844-3859.
[5] Groszyk J, Przyborowski M. Inhibition of the Glycogen Synthase Kinase 3 Family by the Bikinin Alleviates the Long-Term Effects of Salinity in Barley. Int J Mol Sci. 2022 Oct 1;23(19):11644.
[6] Furuya T, Nishihama R, Ishizaki K, et al. A glycogen synthase kinase 3-like kinase MpGSK regulates cell differentiation in Marchantia polymorpha. Plant Biotechnol (Tokyo). 2022 Mar 25;39(1):65-72.
| Cell experiment [1]: | |
Cell lines | Marchantia polymorphawild-type Takaragaike-1 (Tak-1) and proMpCYCD;1:MpCYCD;1Nter-tdTomato-NLS reporter lines. |
Preparation Method | Tak-1 and reporter lines were maintained on half-strength Gamborg's B5 medium supplemented with 1% (w/v) agar and 1% (w/v) sucrose under 50–60μmol m⁻² s⁻¹ continuous white light at 22 °C. Tak-1 were treated with Bikinin at clinically relevant concentrations for indicated durations. |
Reaction Conditions | 25μM or 50μM; 5 days or 10 days |
Applications | Bikinin significantly altered the thallus morphology, resulting in abnormally small and deformed structures. Bikinin also induced the expansion of the meristematic region and enhanced cell proliferation activity, expanding the expression domain of MpCYCD;1 and increasing the area of EdU-incorporated nuclei. |
References: | |
| Cas No. | 188011-69-0 | SDF | |
| Synonyms | Abrasin | ||
| Chemical Name | 4-((5-bromopyridin-2-yl)amino)-4-oxobutanoic acid | ||
| Canonical SMILES | BrC1=CN=C(NC(CCC(O)=O)=O)C=C1 | ||
| Formula | C9H9BrN2O3 | M.Wt | 273.08 |
| Solubility | ≥ 13.65mg/mL in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.6619 mL | 18.3097 mL | 36.6193 mL |
| 5 mM | 732.4 μL | 3.6619 mL | 7.3239 mL |
| 10 mM | 366.2 μL | 1.831 mL | 3.6619 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >99.50% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 11 reference(s) in Google Scholar.)