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C12 NBD Ceramide (d18:1/12:0) (Synonyms: NBD Cer(d18:1/12:0), NBD Ceramide (d18:1/12:0), N-C12-NBD-D-erythro-Sphingosine)

Catalog No.GC43010

C12 NBD Ceramide (d18:1/12:0) is a fluorescent analogue of ceramide, it can be used as a substrate in ceramidase assays.

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C12 NBD Ceramide (d18:1/12:0) Chemical Structure

Cas No.: 202850-01-9

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1mg
$248.00
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5mg
$1,119.00
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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Background

Ceramidase catalyzes the hydrolysis of the N-acyl linkage between the fatty acid and sphingosine base in ceramide to produce sphingosine and a free fatty acid. Three isoforms of ceramidases (acid, neutral, and alkaline) have been characterized based on differences in their catalytic pH optimum. C12 NBD ceramide (d18:1/12:0) is a long-chain fluorescent ceramide analog that can be used for the measurement of alkaline and neutral ceramidase activity. It is hydrolyzed efficiently by alkaline ceramidase from P. aeruginosa and by neutral ceramidase from mouse liver. In addition, alkaline and neutral ceramidases from a variety of mammalian cell lines also hydrolyze C12 NBD ceramide (d18:1/12:0). Acid ceramidase does not appear to utilize C-12 NBD ceramide as a substrate. The possibility exists for C12 NBD ceramide (d18:1/12:0) to also be applied to the measurement of ceramide kinase and/or sphingomyelin synthase activity.

Chemical Properties

Cas No. 202850-01-9 SDF
Synonyms NBD Cer(d18:1/12:0), NBD Ceramide (d18:1/12:0), N-C12-NBD-D-erythro-Sphingosine
Canonical SMILES OC[C@H](NC(CCCCCCCCCCCNC1=CC=C([N+]([O-])=O)C2=NON=C12)=O)[C@H](O)/C=C/CCCCCCCCCCCCC
Formula C36H61N5O6 M.Wt 659.9
Solubility DMF: 30 mg/ml,DMSO: 20 mg/ml,Ethanol: 20 mg/ml Storage Store at -20°C, protect from light
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 1.5154 mL 7.5769 mL 15.1538 mL
5 mM 0.3031 mL 1.5154 mL 3.0308 mL
10 mM 0.1515 mL 0.7577 mL 1.5154 mL
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Average Rating: 5 ★★★★★ (Based on Reviews and 35 reference(s) in Google Scholar.)

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