IF 11
PMID: 37507782 ↗Chlorin e6
Catalog No.: GC43244Purity:>90.00%Synonyms: Ce6
Chlorin e6, a photosensitizer with a simple porphyrin nucleus, has strong absorption peaks at wavelengths of 403nm and 664nm.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 19660-77-6
50mgIn Stock
$56.00
1
100mgIn Stock
$91.00
1
| Pack Size | Price | Availability | Quantity | Action |
|---|---|---|---|---|
| 50mg | $56.00 | In Stock | 1 | |
| 100mg | $91.00 | In Stock | 1 |
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Citations
4 publicationsPublications from researchers using this productDescription
Chlorin e6, a photosensitizer with a simple porphyrin nucleus, has strong absorption peaks at wavelengths of 403nm and 664nm[1]. Chlorin e6 exhibits low dark toxicity, high phototoxicity, and rapid accumulation in target tissues, enabling the generation of singlet oxygen and the formation of intracellular reactive oxygen species (ROS)[2]. Chlorin e6 has been widely used in photodynamic therapy and dynamic acoustic therapy to inhibit tumor growth and regulate bacterial activity[3].
In vitro, Chlorin e6 treatment for 48 hours significantly inhibited the viability of HepG2 and B16-F10 cells, with IC50 values of 1.66µM and 1.67µM, respectively, under 10J/cm2 irradiation (24h)[4]. Chlorin e6 (0.2mg/ml) treatment for 45 minutes combined with 1.0MHz/cm2 ultrasound (60s) induced G2/M phase arrest and apoptosis in SPCA-1 cells[5]. Pretreatment with 170µM Chlorin e6 for 90 minutes, followed by irradiation at 2J/cm2, induced vascular smooth muscle cell death, accompanied by DNA fragmentation, reduced cell membrane polarization, and caspase-3 activation[6].
In vivo, oral administration of Chlorin e6 at a dose of 5mg/kg every 2 days, combined with 4.96mW/cm2 light irradiation for 4 weeks, significantly attenuated high-fat diet-induced obesity, reduced hepatic lipid accumulation, and decreased epididymal adipocyte size in mice[7].
References:
[1] Paul S, Heng P W S, Chan L W. Optimization in solvent selection for chlorin e6 in photodynamic therapy[J]. Journal of fluorescence, 2013, 23(2): 283-291.
[2] Pandit R P, Thapa Magar T B, Shrestha R, et al. Isolation, identification, and biological activities of a new chlorin e6 derivative[J]. International Journal of Molecular Sciences, 2024, 25(13): 7114.
[3] Liao S, Cai M, Zhu R, et al. Antitumor effect of photodynamic therapy/sonodynamic therapy/sono-photodynamic therapy of chlorin e6 and other applications[J]. Molecular pharmaceutics, 2023, 20(2): 875-885.
[4] Huang F, Li Y, Zhang X J, et al. Novel chlorin e6-based conjugates of tyrosine kinase inhibitors: Synthesis and photobiological evaluation as potent photosensitizers for photodynamic therapy[J]. European Journal of Medicinal Chemistry, 2023, 261: 115787.
[5] Chen B, Zheng R, Liu D, et al. The tumor affinity of chlorin e6 and its sonodynamic effects on non-small cell lung cancer[J]. Ultrasonics sonochemistry, 2013, 20(2): 667-673.
[6] Wawrzyńska M, Kałas W, Biały D, et al. In vitro photodynamic therapy with chlorin e6 leads to apoptosis of human vascular smooth muscle cells[J]. Archivum Immunologiae et Therapiae Experimentalis, 2010, 58(1): 67-75.
[7] Shrestha R, Gurung P, Lim J, et al. Anti-obesity effect of Chlorin e6-mediated photodynamic therapy on mice with high-fat-diet-induced obesity[J]. Pharmaceuticals, 2023, 16(7): 1053.
Protocol
| Cell experiment [1]: | |
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Cell lines |
B16-F10 cells |
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Preparation Method |
B16-F10 cells were cultured in RPMI-1640 medium, supplemented with 10% fetal bovine serum (FBS), and 1% penicillin/streptomycin, at 37°C in an incubator with 5% CO2. Cells were seeded into 96-well plates at a density of 5×103 cells/ml and incubated for 24h at 37°C. Cells were exposed to gradient concentrations of Chlorin e6 (0, 0.1, 1, 10, and 100µM) for 48h, cell viability was measured. |
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Reaction Conditions |
0, 0.1, 1, 10, and 100µM; 48h |
|
Applications |
Chlorin e6 treatment reduced cell viability in B16-F10 cells in a dose-dependent manner. |
| Animal experiment [2]: | |
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Animal models |
Male C57BL6 mice |
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Preparation Method |
Male C57BL6 mice (4 weeks old) were housed and maintained in a standard environment (room temperature 20±2°C; humidity 50±5%; light/dark cycle: 12:12h) in the standard lab facility for a week. The mice were randomly divided into 3 groups with similar weights: normal group (Nor), vehicle-treated high-fat diet group (HFD), high-fat diet with Chlorin e6 (5mg/kg) and light of a high fluence rate (4.96mW/cm2). The normal group of mice was fed a normal diet (10% fat), while the remaining groups were fed a high-fat diet (60% fat) for 70 days to increase the body weight by about 70%. Chlorin e6 (5mg/kg/day) was dissolved in normal saline, filtered through a 0.2µm syringe filter, and administered orally to the treated mice every two days with 4.96mW/cm2 light for 4 weeks. The weight changes in mice, along with food and water intake, were measured every week. |
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Dosage form |
5mg/kg; every two days; 4 weeks; p.o. |
|
Applications |
Chlorin e6 treatment combined with 4.96mW/cm2 light decreased body weight in mice with a high-fat diet. |
References: [1] Huang F, Li Y, Zhang X J, et al. Novel chlorin e6-based conjugates of tyrosine kinase inhibitors: Synthesis and photobiological evaluation as potent photosensitizers for photodynamic therapy[J]. European Journal of Medicinal Chemistry, 2023, 261: 115787. [2] Shrestha R, Gurung P, Lim J, et al. Anti-obesity effect of Chlorin e6-mediated photodynamic therapy on mice with high-fat-diet-induced obesity[J]. Pharmaceuticals, 2023, 16(7): 1053. | |
Product Documents
Purity:>90.00%Appearance:A solid
Biological Data
1 / 4
Chemical Properties
CAS No.
19660-77-6
Synonyms
Ce6
Chemical Name
(7S,8S)-3-carboxy-5-(carboxymethyl)-13-ethenyl-18-ethyl-7,8-dihydro-2,8,12,17-tetramethyl-21H,23H-porphine-7-propanoic acid
SMILES
CC1=C2[NH]C(/C(CC(O)=O)=C([C@@H](CCC(O)=O)[C@@H]3C)\[N]=C3/C=C4[NH]/C(C(C=C)=C\4C)=C\C5=[N]/C(C(CC)=C5C)=C\2)=C1C(O)=O
Molecular Formula
C34H36N4O6
Molecular Weight
596.7
Solubility
DMF: 30 mg/ml,DMSO: 30 mg/ml,DMSO:PBS (pH 7.2) (1:6): 0.14 mg/ml,Ethanol: slightly soluble
Storage
Store at -20°C
Shipping Condition
Evaluation samples are shipped with blue ice. Other available sizes are shipped at room temperature or with blue ice upon request.
CalculatorsMolarity / Dilution / Molecular Weight / Unit Conversion / In Vivo Formulation / Solubility
g/mol
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