Coenzyme A (Synonyms: CoA) |
Catalog No.GC43293
One-Click Copy Product Info
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Coenzyme A is an indispensable cofactor in living cells, synthesized from pantothenic acid (vitamin B5), adenosine triphosphate (ATP), and cysteine.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 85-61-0
Sample solution is provided at 25 µL, 10mM.
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Coenzyme A is an indispensable cofactor in living cells, synthesized from pantothenic acid (vitamin B5), adenosine triphosphate (ATP), and cysteine [1]. As an acyl carrier and carbonyl activating group, Coenzyme A plays a crucial role in the oxidation of pyruvate in the citric acid cycle and the metabolism of carboxylic acids (including short-chain and long-chain fatty acids) [2]. Abnormalities in the biosynthesis and homeostasis of Coenzyme A and its derivatives are associated with various human diseases, including cancer, diabetes, and neurodegeneration [3].
In vitro, supplementing Coenzyme A (25μM; 30 days) in the culture medium can significantly restore the function and mitochondrial respiratory activity of neuronal cells from patients with Pantothenate kinase-associated neurodegenerative disease (PKAN), while inhibiting neuronal death and ROS formation [4]. Coenzyme A (1mM; 30min) can completely inhibit the activity of peroxiredoxin 5 (Prdx5), and this inhibitory state can be relieved by dithiothreitol (DTT) reduction [5].
References:
[1] Michal G, Bergmeyer H U. Coenzyme A[M]//Methods of enzymatic analysis. Academic Press, 1974: 1967-1987.
[2] Leonardi R, Zhang YM, Rock CO, Jackowski S. Coenzyme A: back in action. Prog Lipid Res. 2005;44(2-3):125-153.
[3] Tsuchiya Y, Peak-Chew SY, Newell C, et al. Protein CoAlation: a redox-regulated protein modification by coenzyme A in mammalian cells. Biochem J. 2017;474(14):2489-2508. Published 2017 Jul 11.
[4] Orellana DI, Santambrogio P, Rubio A, et al. Coenzyme A corrects pathological defects in human neurons of PANK2-associated neurodegeneration. EMBO Mol Med. 2016;8(10):1197-1211.
[5] Baković J, Yu B Y K, Silva D, et al. A key metabolic integrator, coenzyme A, modulates the activity of peroxiredoxin 5 via covalent modification[J]. Molecular and cellular biochemistry, 2019, 461(1): 91-102.
| Cell experiment [1]: | |
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Cell lines |
Human neuronal precursors cells and neurons (NPCs) |
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Preparation Method |
NPCs from Pantothenate kinase‐associated neurodegeneration (PKAN) patients were seeded on Matrigel‐coated wells and differentiated in medium containing Neurobasal, BDNF (10ng/ml), NT‐3 (10ng/ml), B27, P/S, and doxycycline (2μg/ml). A final concentration of 25μM Coenzyme A was added to treat the cells. Coenzyme A was resuspended in a Neurobasal medium and stored at −20°C. Fresh medium containing Coenzyme A (25μM) was added every 2 days to the cells undergoing treatment. Measure the mitochondrial membrane potential, ROS, the morphology of dendritic branches, and determine the activity of aconitase in neurons. |
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Reaction Conditions |
25μM; 30 days |
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Applications |
Supplementation of Coenzyme A in the culture medium can significantly restore the function of neurons related to PKAN disease and the mitochondrial respiratory activity, preventing neuronal death and ROS formation. |
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References: |
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| Cas No. | 85-61-0 | SDF | |
| Synonyms | CoA | ||
| Canonical SMILES | O[C@H]1[C@H](N2C=NC3=C2N=CN=C3N)O[C@H](COP(OP(OCC(C)(C)[C@@H](O)C(NCCC(NCCS)=O)=O)(O)=O)(O)=O)[C@H]1OP(O)(O)=O | ||
| Formula | C21H36N7O16P3S | M.Wt | 767.5 |
| Solubility | PBS (pH 7.2): 10 mg/ml | Storage | Store at -20°C, protect from light, filled inert atmosphere |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.3029 mL | 6.5147 mL | 13.0293 mL |
| 5 mM | 260.6 μL | 1.3029 mL | 2.6059 mL |
| 10 mM | 130.3 μL | 651.5 μL | 1.3029 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >85.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 11 reference(s) in Google Scholar.)