Coppersensor 1 |
Catalog No.GC62903
One-Click Copy Product Info
|
Coppersensor 1 is a fluorescent sensor for selective and sensitive detection of copper(I) ions (Cu+) in biological samples, including live cells, with an excitation wavelength of 543nm and an emission wavelength of 576nm.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 874748-20-6
Sample solution is provided at 25 µL, 10mM.
Coppersensor 1 is a fluorescent sensor for selective and sensitive detection of copper(I) ions (Cu+) in biological samples, including live cells, with an excitation wavelength of 543nm and an emission wavelength of 576nm[1]. Coppersensor 1 comprising a boron dipyrromethene (BODIPY) chromophore coupled to a thioether-rich receptor, has a picomolar affinity for Cu+ with high selectivity over competing cellular metal ions. CS1 fluorescence increases up to 10-fold on binding to Cu+[2]. Copper is the third most abundant transition metal after iron and zinc, which plays a crucial role in the biological system as it is a cofactor of various human enzymes for pigment production, antioxidant, defense, and other metabolism[3]. Excess copper ions can generate reactive oxygen species (ROS) that damage bio-molecules like proteins, nucleic acids, and lipids, leading to liver, kidney, and digestive disorders, whereas insufficient copper ion intake can lead to metabolic disorders of the brain and nervous system[4][5]. Coppersensor 1 can be applied in the research of cancer, antibacterial mechanisms, and neurodegenerative diseases[6-8].
References:
[1] Miller, E. W., Zeng, L., Domaille, D. W., & Chang, C. J. (2006). Preparation and use of Coppersensor-1, a synthetic fluorophore for live-cell copper imaging. Nature protocols, 1(2), 824–827.
[2] Zeng, L., Miller, E. W., Pralle, A., Isacoff, E. Y., & Chang, C. J. (2006). A selective turn-on fluorescent sensor for imaging copper in living cells. Journal of the American Chemical Society, 128(1), 10–11.
[3] Li, C., Li, Y., & Ding, C. (2019). The Role of Copper Homeostasis at the Host-Pathogen Axis: From Bacteria to Fungi. International journal of molecular sciences, 20(1), 175.
[4] Scolari Grotto, F., & Glaser, V. (2024). Are high copper levels related to Alzheimer's and Parkinson's diseases? A systematic review and meta-analysis of articles published between 2011 and 2022. Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine, 37(1), 3–22.
[5] Madsen, E., & Gitlin, J. D. (2007). Copper and iron disorders of the brain. Annual review of neuroscience, 30, 317–337.
[6] Hu, Y., Qian, Y., Wei, J., Jin, T., Kong, X., Cao, H., & Ding, K. (2021). The Disulfiram/Copper Complex Induces Autophagic Cell Death in Colorectal Cancer by Targeting ULK1. Frontiers in pharmacology, 12, 752825.
[7] Santo, C. E., Quaranta, D., & Grass, G. (2012). Antimicrobial metallic copper surfaces kill Staphylococcus haemolyticus via membrane damage. MicrobiologyOpen, 1(1), 46–52.
[8] Eskici, G., & Axelsen, P. H. (2012). Copper and oxidative stress in the pathogenesis of Alzheimer's disease. Biochemistry, 51(32), 6289–6311.
Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs[1].
1. Preparation of Reagent Stock Solution for Imaging:
(1) Dissolve 0.63mg of solid Coppersensor 1 in each milliliter of DMSO solvent to prepare a 1mM Coppersensor 1 stock solution (MW 630g/mol).
2. Coppersensor 1 Staining of Live Cells:
(1) Cell imaging experiments require a culture period of 1-2 days. Incubate cells according to your normal protocol.
(2) On the day before imaging, passage the cells onto 18mm glass coverslips coated with poly-L-lysine (50μg/mL). Adherent cells for imaging should grow to 50-80% confluence.
(3) Remove the cells from the incubator and transfer a coverslip to a 35mm culture dish containing 3mL of PBS buffer.
(4) Add 15μL of the 1mM Coppersensor 1 stock solution to the culture dish, resulting in a final dye concentration of 5μM. Mix thoroughly.
(The higher the dye concentration, the higher the background fluorescence level)
(5) Incubate in the dark at 25 or 37℃ for 5-20 minutes.
3. Imaging of Coppersensor 1 Stained Cells:
(1) Image under a standard confocal microscope with an excitation wavelength of 543nm and an emission wavelength of 576nm. (Coppersensor 1 can be imaged using any type of fluorescence microscope, including epifluorescence, confocal, and multiphoton).
Precautions:
①It is recommended to set up a positive control group, in which cells are incubated with 30μM oleic acid for 8 hours before proceeding with subsequent experiments.
②Fluorescent dyes are prone to quenching. Please try to avoid light exposure to slow down the quenching of fluorescence.
③For your safety and health, please wear a lab coat and disposable gloves when performing the operations.
References:
[1] Miller, E. W., Zeng, L., Domaille, D. W., & Chang, C. J. (2006). Preparation and use of Coppersensor-1, a synthetic fluorophore for live-cell copper imaging. Nature protocols, 1(2), 824–827.
| Cas No. | 874748-20-6 | SDF | |
| Formula | C30H50BF2N3S4 | M.Wt | 629.81 |
| Solubility | DMSO : 25 mg/mL (39.69 mM; Need ultrasonic) | Storage | Store at 2-8°C,protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.5878 mL | 7.9389 mL | 15.8778 mL |
| 5 mM | 317.6 μL | 1.5878 mL | 3.1756 mL |
| 10 mM | 158.8 μL | 793.9 μL | 1.5878 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >97.50% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 26 reference(s) in Google Scholar.)