5'-GTP trisodium salt |
Catalog No.GC35157
One-Click Copy Product Info
|
5'-GTP trisodium salt is an activator of G protein signaling and a high-energy nucleotide unit for DNA and RNA biosynthesis.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 36051-31-7
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
5'-GTP trisodium salt is an activator of G protein signaling and a high-energy nucleotide unit for DNA and RNA biosynthesis[1]. 5'-GTP trisodium salt is involved in many important physiological processes, such as cell differentiation and metabolic regulation[2]. 5'-GTP trisodium salt is commonly used in studies related to biosynthesis and neurite outgrowth[3][4].
In vitro, 5'-GTP trisodium salt (100μM; 24-96h) induces S-phase arrest, monocytic/macrophagic differentiation (CD11b/CD14 upregulation, NBT positivity), and subsequent apoptosis in human leukemia U937 and KG1 cells[5]. Treatment of human muscle precursor cells with 5'-GTP trisodium salt (500μM; 24h) upregulates myomiRs (miR-133a/b) and Myogenin expression, induces the release of guanosine-loaded exosomes, and promotes early myogenic differentiation[6]. 5'-GTP trisodium salt (0.5mM; 48h) arrests proliferation, induces dendrite-like process extension and strongly increases melanin synthesis in B16 melanoma cells[7].
References:
[1] Wolczyk M, Szymanski J, Trus I, et al. 5' terminal nucleotide determines the immunogenicity of IVT RNAs. Nucleic Acids Res. 2025;53(3):gkae1252.
[2] Guarnieri S, Pilla R, Morabito C, et al. Extracellular guanosine and GTP promote expression of differentiation markers and induce S-phase cell-cycle arrest in human SH-SY5Y neuroblastoma cells. Int J Dev Neurosci. 2009;27(2):135-147.
[3] Kaziro Y. The role of guanosine 5'-triphosphate in polypeptide chain elongation. Biochim Biophys Acta. 1978;505(1):95-127.
[4] Gysbers JW, Guarnieri S, Mariggiò MA, Pietrangelo T, Fanò G, Rathbone MP. Extracellular guanosine 5' triphosphate enhances nerve growth factor-induced neurite outgrowth via increases in intracellular calcium. Neuroscience. 2000;96(4):817-824.
[5] Yazdanparast R, Moosavi MA, Mahdavi M, Lotfi A. Guanosine 5'-triphosphate induces differentiation-dependent apoptosis in human leukemia U937 and KG1 cells. Acta Pharmacol Sin. 2006;27(9):1175-1184.
[6] Pietrangelo T, Di Filippo ES, Locatelli M, et al. Extracellular Guanosine 5'-Triphosphate Induces Human Muscle Satellite Cells to Release Exosomes Stuffed With Guanosine. Front Pharmacol. 2018;9:152.
[7] Giotta GJ, Smith JR, Nicolson GL. Guanosine 5'-triphosphate inhibits growth and stimulates differentiated functions in B16 melanoma cells. Exp Cell Res. 1978;112(2):385-393.
| Cell experiment [1]: | |
Cell lines | human U937 and KG1 cell lines |
Preparation Method | The human U937 and KG1 cell lines were cultured in RPMI-1640 medium supplemented with FBS (10%, v/v), streptomycin (100µg/mL) and penicillin (100U/mL) in CO2 humidified atmosphere at 37ºC. The number of viable cells was estimated by trypan blue exclusion test. Cell number was assessed using a hemocytometer and the percent of growth inhibition was calculated relative to untreated control cells. For cell-cycle analyses, exponentially growing U937 and KG1 cells (1×105cells/well) were treated with 100μM 5'-GTP trisodium salt for 24-96h. After harvesting, the cells were permeabilized with cold ethanol, stained with 500µL PI (50µg/mL in 0.1% Triton X-100/0.1% sodium citrate) and subjected to analyses by flow cytometry. Based on PI staining, the cells with sub-G1 pattern were considered as apoptotic cells. Parallel to nuclear PI fluorescence, the cells size and complexity were also evaluated. According to the light scattering analyses, apoptotic nuclei showed lower forward scatter values along with higher sideward scatter values. Cell debris and small particles were excluded from the analysis by forward/sideward scatter criteria. The population of G0/G1 , S, and G2/M were determined using Multicycle Cell Cycle Software. The results are expressed as the percentage of the cells in each phase. The morphology of cells were studied up to 96h of treatments using a phase-contrast microscope. |
Reaction Conditions | 100μM; 24-96h |
Applications | 5'-GTP trisodium salt induces S-phase arrest, monocytic/macrophagic differentiation in human leukemia U937 and KG1 cells. |
References: | |
| Cas No. | 36051-31-7 | SDF | |
| Canonical SMILES | O[C@@H]([C@H]([C@H](N1C=NC2=C1NC(N)=NC2=O)O3)O)[C@H]3COP(O)(OP(OP(O[Na])(O[Na])=O)(O[Na])=O)=O | ||
| Formula | C10H13N5Na3O14P3 | M.Wt | 589.13 |
| Solubility | Water: ≥ 47 mg/mL (79.78 mM) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.6974 mL | 8.4871 mL | 16.9742 mL |
| 5 mM | 339.5 μL | 1.6974 mL | 3.3948 mL |
| 10 mM | 169.7 μL | 848.7 μL | 1.6974 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 18 reference(s) in Google Scholar.)