Glabranine |
Catalog No.GC36143
One-Click Copy Product Info
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Glabranine is a flavonoid isolated from Tephrosia species. Glabranine inhibits the dengue virus by interacting with the soluble extracellular domain of the DENV2 E protein.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 41983-91-9
Sample solution is provided at 25 µL, 10mM.
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Glabranine is a flavonoid isolated from Tephrosia species. Glabranine inhibits the dengue virus by interacting with the soluble extracellular domain of the DENV2 E protein. Glabranine can be used in research related to dengue virus inhibition, as well as for Glabranine antibacterial and antioxidant activities[1].
In vitro, Glabranine (6–50µM) was used to treat LLC-MK2 cells infected with dengue virus (serotype 2) for 24 hours. Glabranine exhibited no cytotoxicity to the cells and significantly inhibited dengue virus replication[2].
References:
[1] Ismail NA, Jusoh SA. Molecular Docking and Molecular Dynamics Simulation Studies to Predict Flavonoid Binding on the Surface of DENV2 E Protein. Interdiscip Sci. 2017 Dec;9(4):499-511.
[2] Sánchez I, Gómez-Garibay F, Taboada J, et al. Antiviral effect of flavonoids on the dengue virus. Phytother Res. 2000 Mar;14(2):89-92.
| Cell experiment [1]: | |
Cell lines | LLC-MK2 cells (Rhesus monkey kidney epithelial cell line) |
Preparation Method | LLC-MK2 cells were treated with Glabranine at concentrations ranging from 6µM to 50µM for 24 hours. |
Reaction Conditions | 6µM to 50µM; 24h. |
Applications | Glabranine showed no cytotoxicity to LLC-MK2 cells at concentrations up to 50µM and did not affect cell proliferation. In dengue virus (serotype 2)-infected cells, Glabranine significantly inhibited viral replication, reducing dengue virus by 53.9% and 76.9% at concentrations of 12µM and 25µM, respectively. |
References: | |
| Cas No. | 41983-91-9 | SDF | |
| Canonical SMILES | O=C1C2=C(O)C=C(O)C(C/C=C(C)\C)=C2O[C@H](C3=CC=CC=C3)C1 | ||
| Formula | C20H20O4 | M.Wt | 324.37 |
| Solubility | DMSO : 100 mg/mL (308.29 mM; Need ultrasonic) | Storage | Store at -20°C,protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.0829 mL | 15.4145 mL | 30.829 mL |
| 5 mM | 616.6 μL | 3.0829 mL | 6.1658 mL |
| 10 mM | 308.3 μL | 1.5414 mL | 3.0829 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 37 reference(s) in Google Scholar.)