Gamma-Mangostin |
Catalog No.GC38227
One-Click Copy Product Info
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Gamma-Mangostin is a competitive 5-hydroxytryptamine 2A (5-HT2A) receptor antagonist and potent cyclooxygenase-2 (COX-2) inhibitor, as well as a transthyroxin protein (TTR) profibrosis inhibitor.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 31271-07-5
Sample solution is provided at 25 µL, 10mM.
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Gamma-Mangostin is a competitive 5-hydroxytryptamine 2A (5-HT2A) receptor antagonist and potent cyclooxygenase-2 (COX-2) inhibitor, as well as a transthyroxin protein (TTR) profibrosis inhibitor. Gamma-Mangostin binds to the thyroxine (T4)-binding sites and stabilizes the TTR tetramer[1]. Gamma-Mangostin has antibacterial, anticancer, antioxidant properties[2].
In vitro, Gamma-Mangostin inhibited cell proliferation and colony formation in six different colon cancer cell lines, HCT116, SW480, LS174T, RKO, HT29 and DLD1, the 48h IC50 values ranged from 10-15µM for colon cancer cell lines[2]. Gamma-Mangostin inhibited cell proliferation on U87 MG (astrocytoma glioblastoma, grade III) and GBM 8401 (glioblastoma multiforme, grade IV)，the IC50 values of Gamma-Mangostin at 24 hours were 74.14±2.93 and 64.67±2.42μM[3]. Gamma-Mangostin treatment (80μM; 1h) increased intracellular peroxide levels in U87 MG and GBM 8401 cells[3]. Gamma-Mangostin treatment inhibited LPS-induced NO release with IC50 about 6.0µM in RAW264.7 cells[4].
In vivo, treatment of Gamma-Mangostin (5mg/kg; i.p.; 21 days) inhibited tumor xenograft growth in nude mice harboring HCT116 tumor xenografts[2]. Treatment of Gamma-Mangostin (10, and 30mg/kg; single oral dose) markedly improved scopolamine-induced memory impairment in mice[5].
References:
[1]. Chairungsrilerd, Nattaya, et al. "γ-Mangostin, a novel type of 5-hydroxytryptamine 2A receptor antagonist." Naunyn-Schmiedeberg's archives of pharmacology 357.1 (1997): 25-31.
[2]. Krishnamachary, Balaji, et al. "Targeting transcription factor TCF4 by γ-Mangostin, a natural xanthone." Oncotarget 10.54 (2019): 5576.
[3]. Chang, Hui-Fang, et al. "Apoptotic effects of γ-mangostin from the fruit hull of Garcinia mangostana on human malignant glioma cells." Molecules 15.12 (2010): 8953-8966.
[4]. Tewtrakul, Supinya, Chatchai Wattanapiromsakul, and Wilawan Mahabusarakam. "Effects of compounds from Garcinia mangostana on inflammatory mediators in RAW264. 7 macrophage cells." Journal of Ethnopharmacology 121.3 (2009): 379-382.
[5]. Lee, Youngmun, et al. "Inhibition of oxidative neurotoxicity and scopolamine‐induced memory impairment by γ‐Mangostin: in vitro and in vivo evidence." Oxidative medicine and cellular longevity 2019.1 (2019): 3640753.
| Cell experiment [1]: | |
Cell lines | HCT116, SW480, RKO, DLD1, HT29 and LS174T cell lines |
Preparation Method | Cells were grown in 96 well plates and treated with different concentration of Gamma-Mangostin. Proliferation was analyzed by a hexoseaminidase assay. For clonogenicity assay, six-well plates were seeded with 500 viable cells per well and allowed to grow for 24h. The cells were then incubated in the presence of varying concentrations of Gamma-Mangostin for 48h. Gamma-Mangostin containing medium was then removed, and the cells were rinsed in phosphate buffered saline (PBS) and incubated for an additional 10 days in complete medium. The colonies obtained were washed with PBS and fixed in 10% formalin for 10 minutes at room temperature, followed by washing with PBS and staining with Crystal violet. |
Reaction Conditions | 0-40µM; 48h |
Applications | The 50% inhibitory concentration values of Gamma-Mangostin at 48 hours were ranging from 10-15µM for colon cancer cell lines. |
| Animal experiment [2]: | |
Animal models | Six-week-old ICR mice |
Preparation Method | The animals were orally administered with Gamma-Mangostin (5, 10, and 30mg/kg in 40%v/v PEG in water), donepezil (10mg/kg), or vehicle (for control and scopolamine groups). After 30min of administration, memory impairment was induced by intraperitoneal administration of scopolamine (3mg/kg, prepared in normal saline); the control group received normal saline only. Following 30min of scopolamine injection, the acquisition trial was initiated. |
Dosage form | 5, 10, and 30mg/kg; oral; once |
Applications | The groups administered with Gamma-Mangostin at the dosages of 10 and 30mg/kg significantly restored the scopolamine-induced decrease in time latency. |
References: | |
| Cas No. | 31271-07-5 | SDF | |
| Canonical SMILES | O=C1C2=C(OC3=C1C(C/C=C(C)\C)=C(O)C(O)=C3)C=C(O)C(C/C=C(C)\C)=C2O | ||
| Formula | C23H24O6 | M.Wt | 396.43 |
| Solubility | DMSO: 250 mg/mL (630.63 mM) | Storage | Store at 2-8°C |
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.5225 mL | 12.6126 mL | 25.2251 mL |
| 5 mM | 504.5 μL | 2.5225 mL | 5.045 mL |
| 10 mM | 252.3 μL | 1.2613 mL | 2.5225 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
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Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >99.50% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
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Average Rating: 5 (Based on Reviews and 28 reference(s) in Google Scholar.)