Crocin |
Catalog No.GC38423
One-Click Copy Product Info
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Crocin is a water-soluble natural carotenoid derived from the stigma of Crocus sativus.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 42553-65-1
Sample solution is provided at 25 µL, 10mM.
Crocin is a water-soluble natural carotenoid derived from the stigma of Crocus sativus [1]. Crocin significantly inhibits the phosphorylation activity of the JAK2/STAT3 and ERK signaling pathways, thereby blocking STAT3 transcriptional activity and reducing the expression of downstream pro-proliferation genes such as cyclin D1, VEGF, and CXCR4. It also upregulates the pro-apoptotic protein Bax and downregulates the anti-apoptotic protein Bcl-2, ultimately inducing cell cycle arrest (usually at the G0/G1 phase) and triggering mitochondria-dependent apoptotic pathways (caspase-9/caspase-3) [2-3]. Crocin is commonly used to treat various tumors, including colon, skin, thyroid, breast, and gastric cancers [4].
In A549 and SPC-A1 cells, Crocin (0-16mg/mL; 24-72h) inhibited the proliferation of cell in a concentration-dependent manner [5]. In HCT-116 cells, Crocin (0.25-3mg/mL; 48h) possesses significant anti-proliferation effects on human colorectal cancer cells [6]. In BV2 mouse microglial cells, Crocin (0-20μM; 30min) effectively inhibits LPS-induced nitric oxide (NO) release in cultured rat brain microglia [7].
In chronic mild stress (CMS) mice model, animals that received Crocin (4.84mg/kg, 9.69mg/kg, 19.38mg/kg; po; 21d) administration during the three CMS sessions had significantly shorter immobility times during the tail suspension test [8]. In dextran sodium sulfate (DSS)-induced UC mice model, Crocin (10mg/kg, 30mg/kg; ip; 3 weeks) prevents ulcerative colitis by inhibiting NF-κB-mediated inflammation [9].
References:
[1]. Zaghloul M S, Said E, Suddek G M, et al. Crocin attenuates lung inflammation and pulmonary vascular dysfunction in a rat model of bleomycin-induced pulmonary fibrosis[J]. Life sciences, 2019, 235: 116794.
[2]. Yang H, Zhang Y, Zhang D, et al. Crocin exerts anti-tumor effect in colon cancer cells via repressing the JaK pathway[J]. European Journal of Histochemistry: EJH, 2023, 67(3): 3697.
[3]. Boozari M, Hosseinzadeh H. Crocin molecular signaling pathways at a glance: A comprehensive review[J]. Phytotherapy Research, 2022, 36(10): 3859-3884.
[4]. Veisi A, Akbari G, Mard S A, et al. Role of crocin in several cancer cell lines: An updated review[J]. Iranian journal of basic medical sciences, 2020, 23(1): 3.
[5]. Chen S, Zhao S, Wang X, et al. Crocin inhibits cell proliferation and enhances cisplatin and pemetrexed chemosensitivity in lung cancer cells[J]. Translational lung cancer research, 2015, 4(6): 775.
[6]. Aung H H, Wang C Z, Ni M, et al. Crocin from Crocus sativus possesses significant anti-proliferation effects on human colorectal cancer cells[J]. Experimental oncology, 2007, 29(3): 175.
[7]. Nam K N, Park Y M, Jung H J, et al. Anti-inflammatory effects of crocin and crocetin in rat brain microglial cells[J]. European journal of pharmacology, 2010, 648(1-3): 110-116.
[8]. Alsanie W F, Alamri A S, Abdulaziz O, et al. Antidepressant effect of crocin in mice with chronic mild stress[J]. Molecules, 2022, 27(17): 5462.
[9]. Teng S, Hao J, Bi H, et al. The protection of crocin against ulcerative colitis and colorectal cancer via suppression of NF-κB-mediated inflammation[J]. Frontiers in Pharmacology, 2021, 12: 639458.
| Cell experiment [1]: | |
Cell lines | A549 and SPC-A1 cells |
Preparation Method | A549 and SPC-A1 cells were seeded in 96-well plates at a density of 5×104 cells/mL in 100µL medium. Twenty-four hours later, the culture medium was changed to fresh medium with different concentrations of crocin (0, 1, 2, 4, 8 and 16mg/mL). The cells were synchronized by starvation in serum-free medium before treatment with crocin. Each concentration of crocin was added into 5 wells. After 24, 48, and 72h, 20µL MTT (5mg/mL) was added into each well and then incubated for an additional 4h at 37℃. Following removal of the supernatant, 100µL crystalline in dimethyl sulfoxide was added to each well. Absorbance (OD) at 570nm was measured in a microplate reader. |
Reaction Conditions | 0-16mg/mL; 24-72h |
Applications | Crocin inhibited the proliferation of A549 and SPC-A1 cells in a concentration-dependent manner. |
| Animal experiment [2]: | |
Animal models | Chronic mild stress (CMS) mice model |
Preparation Method | All the mice utilized in the study were split into ten groups with eight mice in each group. Carboxy methyl cellulose (CMC) was given to group I as a vehicle, while groups II, III, and IV received doses of Crocin of 4.84mg/kg (low dosage of Crocin—LC), 9.69mg/kg (medium dose of Crocin—MC), and 19.38mg/kg (high dose of Crocin—HC), respectively. The group V animals received imipramine (15mg/kg). For three weeks, all treatments were administered orally 30min prior to the onset of stress (chronic mild stress—CMS) (21 days). On the 21st day, the mice’s locomotor activity was assessed after being exposed to stress for 60min. On the 22nd day, a tail suspension test (TST) was performed on the mice. |
Dosage form | 4.84mg/kg, 9.69mg/kg, 19.38mg/kg; po; 21d |
Applications | Animals that received Crocin administration during the three CMS sessions had significantly shorter immobility times during the TST. |
References: | |
| Cas No. | 42553-65-1 | SDF | |
| Canonical SMILES | O[C@@H]([C@H](O)[C@H]1O)[C@H](O[C@H]1OC(/C(C)=C/C=C/C(C)=C/C=C/C=C(C)/C=C/C=C(C)/C(O[C@@H]([C@@H]([C@@H](O)[C@@H]2O)O)O[C@@H]2CO[C@@H]([C@@H]([C@@H](O)[C@@H]3O)O)O[C@@H]3CO)=O)=O)CO[C@@H]([C@@H]([C@@H](O)[C@@H]4O)O)O[C@@H]4CO | ||
| Formula | C44H64O24 | M.Wt | 976.96 |
| Solubility | DMSO : 125 mg/mL (127.95 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) | Storage | 4°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.0236 mL | 5.1179 mL | 10.2358 mL |
| 5 mM | 204.7 μL | 1.0236 mL | 2.0472 mL |
| 10 mM | 102.4 μL | 511.8 μL | 1.0236 mL |
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
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3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >99.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 5 reference(s) in Google Scholar.)