γ-Tocotrienol |
Catalog No.GD23102
One-Click Copy Product Info
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γ-Tocotrienol (γ-T3) is a subtype of tocotrienol and a major type of vitamin E with two substituted methyl groups.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 14101-61-2
Sample solution is provided at 25 µL, 10mM.
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γ-Tocotrienol (γ-T3) is a subtype of tocotrienol and a major type of vitamin E with two substituted methyl groups[1]. γ-Tocotrienol has effects on anti-inflammation, anti-oxidation, chemoprevention against malignancies (verified in vitro), acute radiation syndrome, and house dust mite-induced asthma [2,3,4].
In vitro studies show that γ-Tocotrienol (0-90μmol/L) inhibited the proliferation of gastric cancer MKN45 cells and AGS cells, and arrested the cell cycle at the G0/G1 phase in a dose-dependent manner. Moreover, autophagy was increased in MKN45 cells treated with γ-Tocotrienol (0-45μmol/L)[2]. γ-Tocotrienol (30μmol/L; 24h) also regulates gastric cancer by targeting notch signaling pathway[5]. And γ-Tocotrienol (PC-3 cells; 10μg/mL; 72h) induces apoptosis in prostate cancer cells by targeting the Ang-1/Tie-2 signaling pathway[6].
In vivo experiments show that γ-Tocotrienol ([18F]F-γ-T-3; 14.8MBq; administration by injection) has upper abdominal accumulation with evidence of renal clearance, only low concentrations in the thorax (lung/heart) and head, and rapid clearance from blood[7]. γ-Tocotrienol also protects mice from targeted thoracic radiation injury(100 or 200mg/kg; 14 or 16Gy/min; SC administration)[8].
References:
[1] Komiyama, K et al. “Studies on the biological activity of tocotrienols.” *Chemical & pharmaceutical bulletin*vol. 37,5 (1989): 1369-71. doi:10.1248/cpb.37.1369
[2] Zhu, Hao et al. “γ-Tocotrienol enhances autophagy of gastric cancer cells by the regulation of GSK3β/β-Catenin pathway.” *Molecular carcinogenesis* vol. 63,10 (2024): 2013-2025. doi:10.1002/mc.23790
[3] Peh, Hong Yong et al. “Vitamin E Isoform γ-Tocotrienol Downregulates House Dust Mite-Induced Asthma.” *Journal of immunology (Baltimore, Md. : 1950)* vol. 195,2 (2015): 437-44. doi:10.4049/jimmunol.1500362
[4] Singh, Vijay K, and Martin Hauer-Jensen. “γ-Tocotrienol as a Promising Countermeasure for Acute Radiation Syndrome: Current Status.” *International journal of molecular sciences* vol. 17,5 663. 3 May. 2016, doi:10.3390/ijms17050663
[5] Xie, Ling, and Juan Yan. “γ-tocotrienol regulates gastric cancer by targeting notch signaling pathway.” *Hereditas* vol. 160,1 15. 13 Apr. 2023, doi:10.1186/s41065-023-00277-w
[6] Tang, Kai Dun et al. “Gamma-Tocotrienol Induces Apoptosis in Prostate Cancer Cells by Targeting the Ang-1/Tie-2 Signalling Pathway.” *International journal of molecular sciences* vol. 20,5 1164. 7 Mar. 2019, doi:10.3390/ijms20051164
[7] Roselt, Peter et al. “Synthesis of [18F]F-γ-T-3, a Redox-Silent γ-Tocotrienol (γ-T-3) Vitamin E Analogue for Image-Based In Vivo Studies of Vitamin E Biodistribution and Dynamics.” *Molecules (Basel, Switzerland)* vol. 25,23 5700. 3 Dec. 2020, doi:10.3390/molecules25235700
[8]Kumar, Vidya P et al. “Gamma Tocotrienol Protects Mice From Targeted Thoracic Radiation Injury.” *Frontiers in pharmacology* vol. 11 587970. 12 Nov. 2020, doi:10.3389/fphar.2020.587970
| Cell experiment [1]: | |
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Cell lines |
human gastric cancer cell line MKN-45 |
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Preparation Method |
MKN-45 cells were seeded into 96-well plates at 5,000 cells per well and allowed to adhere overnight. MKN-45 cells were treated with γ-Tocotrienol (30μmol/L) or not for 24h. Cells were collected and total cellular RNA was isolated using TRIzol reagent. |
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Reaction Conditions |
30μmol/L; 24h |
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Applications |
Significantly altered signaling pathways after γ-Tocotrienol treatment were enriched for human papillomavirus infection (HPV) pathway and notch signaling pathway. The same significantly down-regulated genes notch1 and notch2 were present in both pathways in γ-Tocotrienol-treated gastric cancer cells compared to controls. |
| Animal experiment [2]: | |
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Animal models |
C3H/HeN male mice, irradiated with 14 or 16Gy/min |
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Preparation Method |
For lung-PBI, C3H/HeN male mice were exposed to 14 or 16Gy doses of radiation to the lung area (below the neck to diaphragm). Irradiated mice were administered either saline or γ-Tocotrienol (200mg/kg; SC) 24h prior to PBI. γ-Tocotrienol (200mg/kg) or saline (n=12 per group) were administered SC 24h prior to irradiation. In addition to the irradiated groups of animals, blood and tissues from an age-matched naïve group (n=12) was collected at each time point. |
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Dosage form |
100 or 200mg/kg; SC administration |
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Applications |
Regeneration and increase of cellularity and megakaryocytes on γ-Tocotrienol treatment was compared to significant loss of cellularity in saline group. Peak alveolitis was observed on day 14 post-PBI and protection from alveolitis by γ-Tocotrienol was noted. In irradiated lung tissue, thirty proteins were found to be differentially expressed but modulated by γ-Tocotrienol to reverse the effects of irradiation. |
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References: |
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| Cas No. | 14101-61-2 | SDF | |
| Formula | C28H42O2 | M.Wt | 410.63 |
| Solubility | DMSO : 100 mg/mL (243.53 mM; Need ultrasonic) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.4353 mL | 12.1764 mL | 24.3528 mL |
| 5 mM | 487.1 μL | 2.4353 mL | 4.8706 mL |
| 10 mM | 243.5 μL | 1.2176 mL | 2.4353 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A liquid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)