Cathepsin B from bovine spleen |
Catalog No.GF15486
One-Click Copy Product Info
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Cathepsin B from bovine spleen (Cathepsin B) is a member of the cysteine protease papain-like family. Cathepsin B is synthesized as a preproenzyme consisting of 335 amino acids, with a calculated molecular weight of approximately 36.7kDa. Cathepsin B is localized in the endosomal/lysosomal compartment, where Cathepsin B functions as both an endopeptidase and an exopeptidase. Cathepsin B primarily participates in the routine turnover of intracellular and extracellular proteins, thereby maintaining the homeostatic metabolic activity within the cell.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 9047-22-7
Sample solution is provided at 25 µL, 10mM.
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Cathepsin B from bovine spleen (Cathepsin B) is a member of the cysteine protease papain-like family. Cathepsin B is synthesized as a preproenzyme consisting of 335 amino acids, with a calculated molecular weight of approximately 36.7kDa. Cathepsin B is localized in the endosomal/lysosomal compartment, where Cathepsin B functions as both an endopeptidase and an exopeptidase. Cathepsin B primarily participates in the routine turnover of intracellular and extracellular proteins, thereby maintaining the homeostatic metabolic activity within the cell[1]. Cathepsin B has three major subtypes, including the primary transcript, the primary transcript lacking exon 2, and the primary transcript lacking both exons 2 and 3 [2]. Cathepsin B may also regulate biological processes such as angiogenesis, cell invasion, tumor proliferation, immune resistance, neurogenesis, and cell differentiation. Cathepsin B is frequently associated with the development of inflammatory responses and cancer progression [3, 4].
In vitro, Cathepsin B (10µg/mL) can be taken up by macrophages isolated from the bone marrow of C57Bl/6 mice, and it activates the activity of trypsin in macrophages while inducing inflammatory responses and cell death [5].
In vivo, Cathepsin B (115µg) was instilled into the lungs of hamsters. After 6 weeks, Cathepsin B induced the development of distorted alveolar focal areas and enlarged air space regions in the hamster lungs. Under the microscope, the lungs of hamsters treated with Cathepsin B showed a significantly higher number of secretory cells per millimeter of airway and a significant increase in the total number of cells per millimeter of airway [6-7].
References:
[1] Cavallo-Medved D, Moin K, Sloane B. Cathepsin B: Basis Sequence: Mouse. AFCS Nat Mol Pages. 2011;2011:A000508.
[2] Baudys M, Meloun B, Gan-Erdene T, et al. S-S bridges of cathepsin B and H from bovine spleen: a basis for cathepsin B model building and possible functional implications for discrimination between exo- and endopeptidase activities among cathepsins B, H and L. Biomed Biochim Acta. 1991;50(4-6):569-77.
[3] Mijanović O, Branković A, Panin AN, Savchuk S, Timashev P, Ulasov I, Lesniak MS. Cathepsin B: A sellsword of cancer progression. Cancer Lett. 2019 May 1;449:207-214.
[4] Brun S, Bestion E, Raymond E, et al. GNS561, a clinical-stage PPT1 inhibitor, is efficient against hepatocellular carcinoma via modulation of lysosomal functions. Autophagy. 2022 Mar;18(3):678-694..
[5] Sendler M, Weiss FU, Golchert J, et al. B-Mediated Activation of Trypsinogen in Endocytosing Macrophages Increases Severity of Pancreatitis in Mice. Gastroenterology. 2018 Feb;154(3):704-718.e10.
[6] Cardozo C, Padilla ML, Choi HS, et al. Goblet cell hyperplasia in large intrapulmonary airways after intratracheal injection of cathepsin B into hamsters. Am Rev Respir Dis. 1992 Mar;145(3):675-9.
[7] Lesser M, Padilla ML, Cardozo C. Induction of emphysema in hamsters by intratracheal instillation of cathepsin B. Am Rev Respir Dis. 1992 Mar;145(3):661-8.
| Cell experiment [1]: | |
Cell lines | Macrophages |
Preparation Method | Macrophages were maintained in μm-dishes for 5–7 days in the presence of 20mg/ml Macrophage colony stimulating factor, Co-incubation with freshly Cathepsin B(10μg/mL) was performed over 6 hours. After, cells were washed 3 times with PBS before they were loaded with fluorogen substrates, R110-Ile-Pro-Arg, and/or Suc-Ala-Ala-Pro-Phe-AMC in a concentration of 10 mmol/L for 30 minutes. Cells were washed again carefully and maintained in PBS for microscopy. |
Reaction Conditions | 10μg/mL; 6h |
Applications | Cathepsin B was internalized by macrophages and then Cathepsin B converted to active trypsin intracellularly. |
| Animal experiment [2]: | |
Animal models | Hamsters |
Preparation Method | Hamsters were instilled 115μg of active cathepsin B into the lungs of hamsters three times at 48h intervals. The tissue samples of lung were fixed in 10% neutral formalin and were stained for Hematoxylin-eosin staining. |
Dosage form | 115μg; Intranasal delivery. |
Applications | Cathepsin B-treated hamster large lung intrapulmonary airway contains a significantly greater number of secretory cells per millimeter of airway, with a significant increase in the number of total cells per millimeter of airway. |
References: | |
| Cas No. | 9047-22-7 | SDF | |
| Formula | M.Wt | ||
| Solubility | Storage | Store at -20°C | |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Biological Activity: ≥10 units/mg protein Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)