Home>>Natural Products>>Laurdan

Laurdan

Catalog No.GC18338

Laurdan Chemical Structure

A fluorescent membrane probe

Size Price Stock Qty
10mg
$57.00
In stock
25mg
$134.00
In stock
50mg
$225.00
In stock
100mg
$421.00
In stock

Customer Reviews

Based on customer reviews.

Tel: (626) 353-8530 Email: sales@glpbio.com

Sample solution is provided at 25 µL, 10mM.

Quality Control

Quality Control & SDS

View current batch:

Protocol

Samples

bacteria, cells, and liposomes.

Preparation method

1mg/ml in ethanol, 30mg/ml in DMSO & DMF. Stock solution can be stored below -20℃ for several months.

Reaction Conditions

The excitation wavelength is 360 nm, and emission wavelength is 440 nm and 490 nm. [1]

Applications

Measurements of laurdan generalized polarization (GP) Laurdan is an environment-sensitive fluorescent probe, which is conventionally used for monitoring phase state of lipid membranes. [1] For liposomes, using Laurdan-containing liposomes (Laurdan/lipid molar ratio is 1:200), the vesicle suspension was added to the vesicle suspension containing 40 mM KCl, 50 μM EGTA and 10 mM Tris / HCl (pH 7.5) buffer (final lipid concentration is 50μM), and laurdan fluorescence measured before and after adding various experiments.[1] For cell membranes, the erythrocyte plasma membrane was diluted to 3 ml in 0.01 M phosphate saline buffer. Then Laurdan was added to the membrane to a final concentration of 1 μM, and incubated at 37°C for 60 minutes to incorporate Laurdan into the red blood cell membrane. The steady-state fluorescence intensity of Laurdan was measured at 37°C by a spectrofluorometer. The fluorescence measured in the membrane without Laurdan is always subtracted from the data. Use FLWinLab software to normalize the spectrum. [2] Laurdan fluorescence is measured (λex = 360 nm) by a Cary Eclipse spectrofluorimeter. Emission wavelengths, corresponding to the blue and red peaks of laurdan, were 440 and 490 nm. The generalized polarization (GP) was defined as GP = (I440-I490)/ (I440 + I490), where I440 and I490 are the emission intensities at 440 and 490 nm, respectively. GP can theoretically assume values from +1 (being most ordered) and − 1 (being least ordered).[1]

References:

[1]. Mikhail V. Dubinina, Alena A. Semenovaa, Anna I. Ilzorkinaa, Irina B. Mikheevab, Valery A. Yashinc, Nikita V. Penkovc, Valentina A. Vydrinad, Gumer Yu. Ishmuratovd, Vyacheslav A. Sharapova, Ekaterina I. Khoroshavinaa, Sergey V. Gudkove, Konstantin N. Belosludtsev. Effect of betulin and betulonic acid on isolated rat liver mitochondria and liposomes. BBA – Biomembranes, 2020.1862:183383.

[2]. Vignini Arianna, Alia Sonila, Pugnaloni Sofia, Giulietti Alessia, Bacchetti Tiziana, Mazzanti Laura, Luzzi Simona, Fiorini Rosamaria. Erythrocyte membrane fluidity in mild cognitive impairment and Alzheimer's disease patients. Exp Gerontol. 2019.12;128:110754.

Background

Laurdan is a membrane-permeable fluorescent probe that displays spectral sensitivity to the phospholipid phase of the cell membrane to which it is bound.[1] Quantitation of generalized polarization (GP) of laurdan can be used to identify phospholipid phase. When excited at 340 nm, GP values are 0.6 and -0.2 for gel phase and liquid crystalline phase, respectively. GP does not change with polar head group or pH (in the range 4-10); it changes only with phase state.

Reference:
[1]. Parasassi, T., De Stasio, G., Ravagnan, G., et al. Quantitation of lipid phases in phospholipid vesicles by the generalized polarization of Laurdan fluorescence. Biophys. J. 60(1), 179-189 (1991).

Chemical Properties

Cas No. 74515-25-6 SDF
Synonyms N/A
Chemical Name 1-[6-(dimethylamino)-2-naphthalenyl]-1-dodecanone
Canonical SMILES CN(C)C1=CC=C2C(C=CC(C(CCCCCCCCCCC)=O)=C2)=C1
Formula C24H35NO M.Wt 353.5
Solubility 30mg/ml in DMSO & DMF; Chloroform: 10 mg/ml Storage Store at -20°C, protect from light
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
Shipping Condition Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

  • Molarity Calculator

  • Dilution Calculator

Mass
=
Concentration
x
Volume
x
MW*
 
 
 
**When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / CoA (available online).

Calculate

Reviews

Review for Laurdan

Average Rating: 5 ★★★★★ (Based on Reviews and 29 reference(s) in Google Scholar.)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%
Review for Laurdan

GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.

Required fields are marked with *

You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.