Lysozyme from chicken egg white |
Catalog No.GC33922
One-Click Copy Product Info
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Lysozyme from chicken egg white is a glycoside hydrolase with bactericidal and bacteriostatic properties.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 12650-88-3
Sample solution is provided at 25 µL, 10mM.
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Lysozyme from chicken egg white is a glycoside hydrolase with bactericidal and bacteriostatic properties [1]. Lysozyme hydrolyzes the β(1-4) glycosidic linkage between N-acetylmuramic acid and N-acetylglucosamine found in the peptidoglycan layer of the bacterial cell wall, causing cell lysis [2]. Lysozyme can be used in the cheese-making process to inhibit the growth of spoilage bacteria, preventing various types of cheese from experiencing deterioration in texture, bloating, and off-odors[3]. Lysozyme is widely used as a cell-disrupting agent for the extraction of bacterial intercellular products and as an antibacterial agent in ophthalmologic preparations [4].
Suitable pH: 5.3-6.4. Source: Egg white. Properties: Powder.
Lysozyme is a single peptide chain composed of 129 amino acid residues of 18 types, rich in basic amino acids. Lysozyme contains four disulfide bonds that maintain the enzyme's conformation. Lysozyme is a basic protein with lysine at the N-terminus and leucine at the C-terminus. Lysozyme is a C-type lysozyme and is known to be the most heat-resistant lysozyme.
Definition of Enzyme Activity: a lysozyme activity unit is defined as the 25℃, pH 6.2, under the condition of using gambogic micrococcus suspension at 450nm absorbance change of 0.001 per minute required amount of lysozyme.
Solubility: 0.1M phosphate buffer (pH 6.2), 20mg/ml. Storage conditions: 2-8°C. Purpose: Lysozyme is a kind of alkaline mucopolysaccharide enzyme that hydrolyzes pathogenic bacteria. Lysozyme's primary mechanism involves breaking the β-1,4 glycosidic bonds between N-acetylmuramic acid and N-acetylglucosamine in the bacterial cell wall, thereby decomposing insoluble mucopolysaccharides into soluble glycopeptides, which leads to cell wall rupture, leakage of cellular contents, and bacterial lysis. Lysozyme can directly bind to negatively charged viral proteins, forming complexes with DNA, RNA, and apoproteins, thereby inactivating viruses. Lysozyme is capable of lysing Gram-positive bacteria such as Micrococcus lysodeikticus, Bacillus megaterium, and Sarcina lutea.
References:
[1] Shahmohammadi A. Lysozyme separation from chicken egg white: a review[J]. European Food Research and Technology, 2018, 244(4): 577-593.
[2] Banerjee P, Keener K M, Lukito V D. Influence of carbon dioxide on the activity of chicken egg white lysozyme[J]. Poultry science, 2011, 90(4): 889-895.
[3] Khorshidian N, Khanniri E, Koushki M R, et al. An overview of antimicrobial activity of lysozyme and its functionality in cheese[J]. Frontiers in nutrition, 2022, 9: 833618.
[4] Su C K, Chiang B H. Partitioning and purification of lysozyme from chicken egg white using aqueous two-phase system[J]. Process Biochemistry, 2006, 41(2): 257-263.
| Cas No. | 12650-88-3 | SDF | |
| Formula | M.Wt | ||
| Solubility | Water : 10 mg/mL | Storage | -20°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Biological Activity: ≥20000U/mg Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 16 reference(s) in Google Scholar.)