Neoeriocitrin (Synonyms: Eriodictyol 7-O-neohesperidoside) |
Catalog No.GC40847
One-Click Copy Product Info
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Neoeriocitrin is a highly potent inhibitor of lactate dehydrogenase A, with an IC50 value of 11.198 ± 0.90µM.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 13241-32-2
Sample solution is provided at 25 µL, 10mM.
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Neoeriocitrin is a highly potent inhibitor of lactate dehydrogenase A, with an IC50 value of 11.198 ± 0.90µM [1]. Neoeriocitrin has an inhibition effect on pyruvate kinase isoenzyme M2 at a very low concentration of 0.65mM[2]. Neoeriocitrin has been widely used in enzyme inhibition studies and anti-inflammatory experiments[3].
In vitro, Neoeriocitrin treatment of MC3T3-E1 cells at a concentration of 2μg/ml for 72 hours showed great proliferation and osteogenic differentiation effects, Neoeriocitrin significantly enhanced the alkaline phosphatase (ALP) activity of MC3T3-E1 cells, and up-regulated the expression of Runx2, COLI, and OCN[4]. Treatment of human cartilage explants with Neoeriocitrin at a concentration of 50µg/mL for 120h significantly inhibited IL-1β-induced NO production, inhibited proteolytic enzymes (especially matrix metalloproteinase-13), prevented glycosaminoglycan (GAG) release, and reduced structural and mechanical damage to cartilage[5]. Pretreatment of INS-1E cells with Neoeriocitrin (1µM) for 21 hours increased cell viability and reduced the number of apoptotic cells induced by streptozotocin[6].
References:
[1] Yırtıcı Ü. Natural flavonoids as promising lactate dehydrogenase A inhibitors: Comprehensive in vitro and in silico analysis[J]. Archiv der Pharmazie, 2024, 357(9): 2400455.
[2] Aslan E, Guler C, Adem S. In vitro effects of some flavonoids and phenolic acids on human pyruvate kinase isoenzyme M2[J]. Journal of enzyme inhibition and medicinal chemistry, 2016, 31(2): 314-317.
[3] Ahn, J.S.; Lee, C.H.; Liu, X.-Q.; Hwang, K.W.; Oh, M.H.; Park, S.-Y.; Whang, W.K. Neuroprotective Effects of Phenolic Constituents from Drynariae Rhizoma. Pharmaceuticals 2024, 17, 1061.
[4] Li L, Zeng Z, Cai G. Comparison of neoeriocitrin and naringin on proliferation and osteogenic differentiation in MC3T3-E1[J]. Phytomedicine, 2011, 18(11): 985-989.
[5] Crascì L, Panico A. Protective effects of many citrus flavonoids on cartilage degradation process[J]. Journal of Biomaterials and Nanobiotechnology, 2013, 4(3): 279-283.
[6] Aldemir E E, Selmanoğlu G, Karacaoğlu E. The Potential Protective Role of Neoeriocitrin in a Streptozotocin-Induced Diabetic Model in INS-1E Pancreatic ß-Cells[J]. Brazilian Archives of Biology and Technology, 2022, 66: e23220138.
| Cell experiment [1]: | |
Cell lines | MC3T3-E1 cells |
Preparation Method | The MC3T3-E1 cells were cultured inα-Modified minimal essential medium (α-MEM) supplemented with 10% fetal bovine serum (FBS), 100U/ml penicillin, and 100μg/ml streptomycin at 37°C with 5%CO2. The medium was changed twice a week. MC3T3-E1 cells were harvested by trypsin treatment and suspended in culture medium, and seeded into 96-well dishes at a density of 5×104cells /ml. After 24 hours, the medium was replaced with a new medium containing 5% FBS and osteogenic supplement (OS): consisting of 10μg/ml ascorbic acid and 10mmol/L β-glycerophosphoric acid. Differentiation was induced by Neoeriocitrin at different concentrations (0, 2, 4, 6, 8, 10, 12, 14, 16, 18, 20μg/ml). After 72h of incubation, cell proliferation was measured by MTT assay. The assay is based on the ability of living cells to convert soluble 3-(4, 5-dimethyl-thiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) into insoluble dark blue methylene dye reaction products. MTT was dissolved in PBS at a concentration of 5g/L and passed through a 0.22mm filter to remove bacteria. The MTT stock solution was added to the culture medium at a volume ratio of 1:10, incubated at 37°C for 4h, and then DMSO was added to mix to dissolve the dark blue crystals, which were left at room temperature for 10 min before reading with a microplate reader at a wavelength of 492nm. |
Reaction Conditions | 0, 2, 4, 6, 8, 10, 12, 14, 16, 18, 20μg/ml; 72h |
Applications | Neoeriocitrin at the concentration of 2μg/ml showed an excellent effect in promoting proliferation and bone differentiation. |
References: | |
| Cas No. | 13241-32-2 | SDF | |
| Synonyms | Eriodictyol 7-O-neohesperidoside | ||
| Canonical SMILES | O[C@H]1[C@H](O)[C@@H](CO)O[C@@H](OC2=CC(O)=C(C(C[C@@H](C3=CC=C(O)C(O)=C3)O4)=O)C4=C2)[C@@H]1O[C@@]5([H])[C@H](O)[C@H](O)[C@@H](O)[C@H](C)O5 | ||
| Formula | C27H32O15 | M.Wt | 596.5 |
| Solubility | DMF: 15 mg/ml,DMSO: 30 mg/ml,DMSO:PBS(pH 7.2) (1:4): 0.2 mg.ml,Ethanol: 1 mg/ml | Storage | Store at -20°C,protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.6764 mL | 8.3822 mL | 16.7645 mL |
| 5 mM | 335.3 μL | 1.6764 mL | 3.3529 mL |
| 10 mM | 167.6 μL | 838.2 μL | 1.6764 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 4 reference(s) in Google Scholar.)