Ochratoxin C (Synonyms: Ochratoxin A ethyl ester) |
Catalog No.GC44485
One-Click Copy Product Info
|
Ochratoxin C (OTC) is a derivative of ochratoxin, a type of fungal toxin, and belongs to the ethyl ester derivative of Ochratoxin A.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 4865-85-4
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
Ochratoxin C (OTC) is a derivative of ochratoxin, a type of fungal toxin, and belongs to the ethyl ester derivative of Ochratoxin A. Ochratoxin is mainly produced by Penicillium and Aspergillus genera[1].
In vitro, in THP-1 cells, exposure to Ochratoxin C (1ng/mL) for 15 days increased mitochondrial activity and IL-6 production, while impairing membrane integrity and inhibiting cell proliferation together with TNF-α and IL-8 production[2]. Treatment of PBMCs with Ochratoxin C (0.46-3000ng/mL) for 4-72h inhibited cell proliferation in a dose-dependent manner with an IC₅₀ of 300ng/mL, and significantly reduced intracellular free radical and TNF-α production at concentrations of 1-10ng/mL[3]. Ochratoxin C exhibited an LC₅₀ of 0.009mM in HeLa cells[4].
In vivo, Ochratoxin C was lethal to 6-month-old Mt. Shasta strain rainbow trout following intraperitoneal administration, with an LD₅₀ of 3mg/kg after a 10-day observation period[5].
References:
[1] Ringot D, Chango A, Schneider YJ, Larondelle Y. Toxicokinetics and toxicodynamics of ochratoxin A, an update. Chem Biol Interact. 2006;159(1):18-46.
[2] Köhler H, Heller M, Erler W, Müller G. Effects of a long-term exposure with OTA or OTC on functions of a human monocytic cell line. Mycotoxin Res. 2003;19(2):108-112.
[3]Köhler H, Heller M, Erler W, Müller G, Rosner H, Gräfe U. Effect of ochratoxin A and ochratoxin C on the monocyte and lymphocyte function. Mycotoxin Res. 2002;18 Suppl 2:169-172.
[4] Xiao H, Madhyastha S, Marquardt RR, et al. Toxicity of ochratoxin A, its opened lactone form and several of its analogs: structure-activity relationships. Toxicol Appl Pharmacol. 1996;137(2):182-192.
[5] Doster RC, Sinnhuber RO, Pawlowski NE. Acute intraperitoneal toxicity of ochratoxin A and B derivatives in rainbow trout (Salmo gairdneri). Food Cosmet Toxicol. 1974;12:499-505.
| Cell experiment [1]: | |
Cell lines | THP-1 cell |
Preparation Method | Cells were propagated in 24-well cell culture plates for 15 days. Ochratoxin C (1ng/mL) was included in the cell culture medium during the whole cultivation period. At the end of the exposure time, parameters of cell viability and cell function were examined. |
Reaction Conditions | 1ng/mL; 15d |
Applications | After 15 days of exposure to Ochratoxin C, mitochondrial activity and the production of IL-6 were increased, cell membrane integrity was disturbed, proliferation and the production of TNF-αand IL-8 were inhibited. |
References: | |
| Cas No. | 4865-85-4 | SDF | |
| Synonyms | Ochratoxin A ethyl ester | ||
| Canonical SMILES | O=C1O[C@H](C)CC2=C1C(O)=C(C(N[C@H](C(OCC)=O)CC3=CC=CC=C3)=O)C=C2Cl | ||
| Formula | C22H22ClNO6 | M.Wt | 431.9 |
| Solubility | DMF: Soluble,DMSO: Soluble,Ethanol: Soluble,Methanol: Soluble | Storage | Store at -20°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.3154 mL | 11.5768 mL | 23.1535 mL |
| 5 mM | 463.1 μL | 2.3154 mL | 4.6307 mL |
| 10 mM | 231.5 μL | 1.1577 mL | 2.3154 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 32 reference(s) in Google Scholar.)