Sample solution is provided at 25 µL, 10mM.
GlpBio Products Cited In Reputable Papers
Papain Inhibitor peptide,(C19H29N7O6),a peptide with the sequence H-Gly-Gly-Tyr-Arg-OH,MW= 451.4,this peptide Inhibits the peptidase activity of Papain. Papain,also known as papaya proteinase I,is a cysteine protease enzyme present in papaya and mountain papaya. The mechanism by which it breaks peptide bonds involves deprotonation of Cys-25 by His-159. Asparagine-175 helps to orient the imidazole ring of His-159 to allow this deprotonation to take place. Cys-25 then performs a nucleophilic attack on the carbonyl carbon of a peptide backbone. This frees the amino terminal of the peptide,and forms a covalent acyl-enzyme intermediate. The enzyme is then deacylated by a water molecule,and releases the carboxy terminal portion of the peptide. In immunology,papain is known to cleave the Fc (crystallisable) portion of immunoglobulins (antibodies) from the Fab (antigen-binding) portion1. Papain prefers to cleave at: (hydrophobic) -(Arg or Lys)- cleaves here -(not Val). Hydrophobic is Ala,Val,Leu,Ile,Phe,Trp,or Tyr2. Papain can be used to dissociate cells in the first step of cell culture preparations. It is also used as an ingredient in various enzymatic debriding preparations,notably Accuzyme. These are used in the care of some chronic wounds to clean up dead tissue.Papain can also be found as an ingredient in some toothpastes or mints as teeth-whitener.
1. Rawlings ND,Barrett AJ (1994). "Families of cysteine peptidases". Meth. Enzymol. 244: 461–486.
2. Lopes MC,Mascarini RC,da Silva BM,Flório FM,Basting RT (2007). "Effect of a papain-based gel for chemomechanical caries removal on dentin shear bond strength". J Dent Child (Chic) 74 (2): 93–7.
|Chemical Name||(2S)-2-[[(2R)-2-[[2-[(2-aminoacetyl)amino]acetyl]amino]-3-(4-hydroxyphenyl)propanoyl]amino]-5-(diaminomethylideneamino)pentanoic acid|
|Solubility||≥ 45.1 mg/mL in DMSO, ≥ 90.4 mg/mL in H2O||Storage||Store at -20°C|
|General tips||For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.|
|Shipping Condition||Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
In vivo Formulation Calculator (Clear solution)
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Average Rating: 5(Based on Reviews and 9 reference(s) in Google Scholar.)
GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
Required fields are marked with *