Puromycin dihydrochloride (Synonyms: CL13900) |
Catalog No.GC16384
One-Click Copy Product Info
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Puromycin dihydrochlorideis a naturally occurring aminonucleoside antibiotic that mimics the 3′ adenosine of a tRNA charged with a modified tyrosine.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 58-58-2
Sample solution is provided at 25 µL, 10mM.
Puromycin dihydrochloride is a naturally occurring aminonucleoside antibiotic that mimics the 3′ adenosine of a tRNA charged with a modified tyrosine[1]. Puromycin dihydrochloride inhibits protein synthesis by ribosome-catalyzed incorporation into the C-terminus of elongating nascent chains, blocking further extension and resulting in premature termination of translation[2]. Puromycin dihydrochloride has been widely used in gene regulation research to regulate the mRNA stability of genes with nonsense mutations and to screen cells with resistance genes[3].
In vitro, Puromycin dihydrochloride treatment (20μM) for 3 days induced apoptosis and endoplasmic reticulum stress in J774A.1 cells, and caused an increase in the level of polyubiquitination of proteins[4].
References:
[1] Enam S U, Zinshteyn B, Goldman D H, et al. Puromycin reactivity does not accurately localize translation at the subcellular level[J]. Elife, 2020, 9: e60303.
[2] Aviner R. The science of puromycin: From studies of ribosome function to applications in biotechnology[J]. 2020.
[3] Su L H, Lee G A, Huang Y C, et al. Neomycin and puromycin affect gene expression in Giardia lamblia stable transfection[J]. Molecular and Biochemical Parasitology, 2007, 156(2): 124-135.
[4] Croons V, Martinet W, Herman A G, et al. Differential effect of the protein synthesis inhibitors puromycin and cycloheximide on vascular smooth muscle cell viability[J]. The Journal of Pharmacology and Experimental Therapeutics, 2008, 325(3): 824-832.
| Cell experiment [1]: | |
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Cell lines |
J774A.1 cells |
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Preparation Method |
J774A.1 cells were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS), penicillin (100U/ml) and streptomycin (100µg/ml) at 37°C in an incubator with 5% CO2. Cells (5×103/well) were seeded in 96-well plates and cultured at 37°C in 5% CO2 overnight and then treated with different concentrations of Puromycin dihydrochloride (0, 0.1, 1, and 10μM) for 72h, cell viability was assessed. |
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Reaction Conditions |
0, 0.1, 1, and 10μM; 72h |
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Applications |
Puromycin dihydrochloride treatment reduced cell viability of J774A.1 cells in a dose-dependent manner. |
References: [1] Croons V, Martinet W, Herman A G, et al. Differential effect of the protein synthesis inhibitors puromycin and cycloheximide on vascular smooth muscle cell viability[J]. The Journal of Pharmacology and Experimental Therapeutics, 2008, 325(3): 824-832. | |
| Cas No. | 58-58-2 | SDF | |
| Synonyms | CL13900 | ||
| Chemical Name | (S)-2-amino-N-((2S,3R,4S,5R)-5-(6-(dimethylamino)-9H-purin-9-yl)-4-hydroxy-2-(hydroxymethyl)tetrahydrofuran-3-yl)-3-(4-methoxyphenyl)propanamide dihydrochloride | ||
| Canonical SMILES | O[C@H]1[C@H]([C@@H](CO)O[C@H]1N2C3=NC=NC(N(C)C)=C3N=C2)NC([C@H](CC(C=C4)=CC=C4OC)N)=O.Cl.Cl | ||
| Formula | C22H29N7O5.2HCl | M.Wt | 544.43 |
| Solubility | ≥ 27.2mg/mL in DMSO, ≥ 99.4mg/mL in Water | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.8368 mL | 9.1839 mL | 18.3678 mL |
| 5 mM | 367.4 μL | 1.8368 mL | 3.6736 mL |
| 10 mM | 183.7 μL | 918.4 μL | 1.8368 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 35 reference(s) in Google Scholar.)