Salicylic Acid-d4 (Synonyms: 2-Hydroxybenzoic Acid-d4)

Salicylic acid-d₄ is intended for use as an internal standard for the quantification of salicylic acid by GC- or LC-MS. Salicylic acid is an active metabolite of the COX inhibitor aspirin and a plant hormone that has been found in Cucumis sativus.^1,2 It protects Cucumis sativus leaves from infection by S. fuliginea or E. cichoracearum when used at a concentration of 10 mM, as well as inhibits A. thaliana root growth at 0.3 mM.^2,3 Salicylic acid (1 and 4 mM) reduces IFN-γ- and TNF-α-induced increases in NOS2 expression in primary rat cardiac fibroblasts.⁴ It decreases urine prostaglandin E₂ levels in dogs when administered at a dose of 40 mg/kg.⁵

1.Klessig, D.F.Newly identified targets of aspirin and its primary metabolite, salicylic acidDNA Cell Biol.35(4)163-166(2016) 2.Feussner, I., Fritz, I.G., Hause, B., et al.Induction of a new lipoxygenase form in cucumber leaves by salicylic acid or 2,6-dichloroisonicotinic acidBot. Acta110(2)101-108(1997) 3.Kim, T.-H., Kunz, H.-H., Bhattacharjee, S., et al.Natural variation in small molecule-induced TIR-NB-LRR signaling induces root growth arrest via EDS1- and PAD4-complexed R protein VICTR in ArabidopsisPlant Cell24(12)5177-5192(2012) 4.Farivar, R.S., and Brecher, P.Salicylate is a transcriptional inhibitor of the inducible nitric oxide synthase in cultured cardiac fibroblastsJ. Biol. Chem.271(49)31585-31592(1996) 5.Zambraski, E.J., Atkinson, D.C., and Diamond, J.Effects of salicylate vs. aspirin on renal prostaglandins and function in normal and sodium-depleted dogsJ. Pharmacol. Exp. Ther.247(1)96-103(1988)