SIH (Salinazid) (Synonyms: NSC 33760, Salicylaldehyde Isonicotinoyl Hydrazine, Salinazid) |
Catalog No.GC48894
One-Click Copy Product Info
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SIH (Salinazid) is a lipophilic tridentate iron chelator with anti-tuberculosis activity.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 495-84-1
Sample solution is provided at 25 µL, 10mM.
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SIH (Salinazid) is a lipophilic tridentate iron chelator with anti-tuberculosis activity. SIH exerts antioxidant effects by chelating iron ions, activates the Nrf2 signaling pathway to reduce oxidative stress, and exhibits moderate cytotoxicity against tumor cells. SIH can be used in research related to iron overload diseases, cardioprotection, radiation protection, tumors, and tuberculosis[1-4].
In vitro, K562 cells were treated with SIH (100μM) for 10 minutes. SIH significantly reduced the level of the labile iron pool (LIP)[5]. HepG2 cells were treated with SIH (10μM) for 24 hours. SIH significantly increased intracellular reactive oxygen species levels, activated the Nrf2 signaling pathway, and increased glutathione concentration[6].
In vivo, C57BL/6 mice were intravenously administered a single dose of SIH (6mg/kg) 30 minutes before intraperitoneal injection of a lethal dose of Fas antibody (0.5mg/kg). SIH significantly reduced plasma aspartate aminotransferase levels and extended survival time[7]. SOD1G37R transgenic mice were intraperitoneally injected with SIH (50mg/kg; once or twice per week) for 52 weeks. SIH significantly extended the lifespan of the mice, increased the survival rate of spinal motor neurons, and improved motor function[8].
References:
[1] Georgieva N, Gadjeva V, Tolekova A, et al. Hepatoprotective effect of isonicotinoylhydrazone SH7 against chronic isoniazid toxicity. Pharmazie. 2005 Feb;60(2):138-41.
[2] Condeles AL, Toledo Junior JC. The Labile Iron Pool Reacts Rapidly and Catalytically with Peroxynitrite. Biomolecules. 2021 Sep 9;11(9):1331.
[3] Tsafack A, Loyevsky M, Ponka P, et al. Mode of action of iron (III) chelators as antimalarials. IV. Potentiation of desferal action by benzoyl and isonicotinoyl hydrazone derivatives. J Lab Clin Med. 1996 Jun;127(6):574-82.
[4] Evans AT, Croft SL, Peters W, et al. Antileishmanial effects of clofazimine and other antimycobacterial agents. Ann Trop Med Parasitol. 1989 Oct;83(5):447-54.
[5] Konijn AM, Glickstein H, Vaisman B, et al. The cellular labile iron pool and intracellular ferritin in K562 cells. Blood. 1999 Sep 15;94(6):2128-34.
[6] Caro AA, Commissariat A, Dunn C, et al. Prooxidant and antioxidant properties of salicylaldehyde isonicotinoyl hydrazone iron chelators in HepG2 cells. Biochim Biophys Acta. 2015 Nov;1850(11):2256-64.
[7] Lesnikov VA, Abbasi N, Lesnikova MP, et al. Protection of human and murine hepatocytes against Fas-induced death by transferrin and iron. Apoptosis. 2006 Jan;11(1):79-87.
[8] Jeong SY, Rathore KI, Schulz K, et al. Dysregulation of iron homeostasis in the CNS contributes to disease progression in a mouse model of amyotrophic lateral sclerosis. J Neurosci. 2009 Jan 21;29(3):610-9.
| Cell experiment [1]: | |
Cell lines | HepG2 cells (human hepatoma cell line) |
Preparation Method | HepG2 cells were maintained in Eagle's minimal essential medium (MEM) supplemented with 10% fetal bovine serum at 37°C, 5% CO₂. Cells were treated with SIH (10μM) for 24h. |
Reaction Conditions | 10μM; 24h |
Applications | SIH significantly increased intracellular reactive oxygen species (ROS) levels, activated the Nrf2 signaling pathway, and increased glutathione concentration. |
| Animal experiment [2]: | |
Animal models | SOD1G37R transgenic mice (on a C57BL/6 background) |
Preparation Method | Mice were injected intraperitoneally with SIH (50mg/kg) starting at 8 months of age, either once or twice per week. Control mice received vehicle (PBS). Survival analysis was performed for 52 weeks, and mice were sacrificed at 50 weeks of age (end stage for vehicle-treated mice) for histological analysis of motor neuron survival in the lumbar spinal cord. |
Dosage form | 50mg/kg; i.p.; once or twice per week for 52 weeks |
Applications | SIH treatment extended life span by 5 weeks, accompanied by increased survival of spinal motor neurons and improved locomotor function. |
References: | |
| Cas No. | 495-84-1 | SDF | |
| Synonyms | NSC 33760, Salicylaldehyde Isonicotinoyl Hydrazine, Salinazid | ||
| Canonical SMILES | O=C(C1=CC=NC=C1)NN=CC2=C(O)C=CC=C2 | ||
| Formula | C13H11N3O2 | M.Wt | 241.2 |
| Solubility | DMF: 10 mg/ml,DMF:PBS (pH 7.2) (1:3): 0.23 mg/ml,DMSO: 5 mg/ml | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.1459 mL | 20.7297 mL | 41.4594 mL |
| 5 mM | 829.2 μL | 4.1459 mL | 8.2919 mL |
| 10 mM | 414.6 μL | 2.073 mL | 4.1459 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 12 reference(s) in Google Scholar.)