Spinosyn D (Synonyms: A 83543D, Lepicidin D) |
Catalog No.GC44938
One-Click Copy Product Info
|
Spinosyn D is a member of the spinosyn family of insecticides, derived from the fermentation of the soil actinomycete Saccharopolyspora spinosa with DT50 value of 14.5 days when incubated aerobically in moist soil.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 131929-63-0
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
Spinosyn D is a member of the spinosyn family of insecticides, derived from the fermentation of the soil actinomycete Saccharopolyspora spinosa with DT50 value of 14.5 days when incubated aerobically in moist soil[1]. Spinosyn D is a minor component of commercial insecticide spinosad,along with its more abundant counterpart Spinosyn A[2].
In vivo, [14C]Spinosyn D was orally administrated into (25mg/day) goats for 3 days by capsule. Within 24 hours of the last dose, 20% for Spinosyn D was found in the tissues and milk - predominantly the fat, and excretion of the 14C was mainly via the faeces, 76% from Spinosyn D were found in excreta and intestine. 0.94mg [14C]Spinosyn D was dosed orally by capsule to 10 Leghorn laying hens, each weighing 1.4kg, daily for 5 days. Within 24 hours of the last dose, for Spinosyn D approximately 82% of the applied 14C was accounted for (78% in the excreta, 0.6% in eggs, 0.8% in fat, 1.2% in liver, 0.5% in muscle and 0.2% in kidney)[3]. The persistence and residual toxicity of Spinosyn D was evaluated on tomato foliage. Initial residues were 59.12mg/g when applied at 100% maximum field registered concentration (MFRC) (720mg/L active ingredient) and 45.88mg/g at 50% MFRC. After 40 days, residues decreased to 20.71mg/g and 15.39mg/g, respectively. Spinosyn D caused less 10% mortality in E. varians adults within 10 days of exposure to treated tomato leaves, with no mortality from older residues[4].
References:
[1] Saunders DG, Bret BL. Fate of spinosad in the environment. Down to Earth.1997.52: 14–20.
[2] Zhao C, Huang Y, Guo C, et al. Heterologous Expression of Spinosyn Biosynthetic Gene Cluster in Streptomyces Species Is Dependent on the Expression of Rhamnose Biosynthesis Genes. J Mol Microbiol Biotechnol. 2017;27(3):190-198.
[3] Kirst H A. The spinosyn family of insecticides: realizing the potential of natural products research. J Antibiot (Tokyo). 2010 Mar;63(3):101-11.
[4] Morales S, Martínez A M, Figueroa J I, et al. Foliar persistence and residual activity of four insecticides of different mode of action on the predator Engytatus varians (Hemiptera: Miridae). Chemosphere. 2019 Nov:235:76-83.
| Animal experiment [1]: | |
Animal models | Goat |
Preparation Method | The goats were dosed orally for 3 days by capsule with a nominal 25mg/day [14C]Spinosyn D equivalent to 10 ppm in the feed, The feed intake was 2.5-3.0kg/animal/day, and the milk averages 0.98 and 1.36kg per day. The milk and excreta were collected throughout the study and the goats slaughtered within 24 hours of the last dose. Tissue samples (composites of equal amounts of longissimus dorsi, semimembranosus and triceps muscles, and of omental and perirenal fat, and kidneys and liver) were analysed. |
Dosage form | 25mg/day; oral administration; 3 days |
Applications | A considerable proportion of the residues, 20% for Spinosyn D, was found in the tissues and milk - predominantly the fat, and excretion of the 14C was mainly via the faeces, 76% from Spinosyn D were found in excreta and intestine. |
[1] Kirst H A. The spinosyn family of insecticides: realizing the potential of natural products research. J Antibiot (Tokyo). 2010 Mar;63(3):101-11. | |
| Cas No. | 131929-63-0 | SDF | |
| Synonyms | A 83543D, Lepicidin D | ||
| Canonical SMILES | CN(C)[C@H]1CC[C@@](O[C@H]([C@@H](C)C2=O)CCC[C@H](CC)OC(C[C@]3([H])C2=C[C@]4([H])[C@@]3([H])C=C(C)[C@@]5([H])[C@@]4([H])C[C@H](O[C@]6([H])O[C@@H](C)[C@H](OC)[C@@H](OC)[C@H]6OC)C5)=O)([H])O[C@@H]1C | ||
| Formula | C42H67NO10 | M.Wt | 746 |
| Solubility | DMF: Soluble,DMSO: Soluble,Ethanol: Soluble,Methanol: Soluble | Storage | Store at -20°C; protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.3405 mL | 6.7024 mL | 13.4048 mL |
| 5 mM | 268.1 μL | 1.3405 mL | 2.681 mL |
| 10 mM | 134 μL | 670.2 μL | 1.3405 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL saline, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >95.00% Appearance: A solid
- COA (Certificate of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 35 reference(s) in Google Scholar.)